Validation of RNAi by real time PCR

Knud Josefsen; Ying Chiu Lee

doi:10.1007/978-1-59745-248-9_14

Validation of RNAi by real time PCR

Knud Josefsen, Ying Chiu Lee

Afdeling for Patologi DIA

1 Citationer (Scopus)

Abstract

Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.

Originalsprog	Engelsk
Tidsskrift	Methods in Molecular Biology
Vol/bind	703
Sider (fra-til)	205-17
Antal sider	13
ISSN	1064-3745
DOI	https://doi.org/10.1007/978-1-59745-248-9_14
Status	Udgivet - 2011

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10.1007/978-1-59745-248-9_14

Citationsformater

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title = "Validation of RNAi by real time PCR",

abstract = "Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.",

keywords = "DNA Primers, Gene Expression, HeLa Cells, Humans, Luciferases, Organic Chemicals, Polymerase Chain Reaction, RNA Interference",

author = "Knud Josefsen and Lee, {Ying Chiu}",

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AU - Josefsen, Knud

AU - Lee, Ying Chiu

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AB - Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.

KW - DNA Primers

KW - Gene Expression

KW - HeLa Cells

KW - Humans

KW - Luciferases

KW - Organic Chemicals

KW - Polymerase Chain Reaction

KW - RNA Interference

U2 - 10.1007/978-1-59745-248-9_14

DO - 10.1007/978-1-59745-248-9_14

M3 - Journal article

C2 - 21125492

SN - 1064-3745

VL - 703

SP - 205

EP - 217

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Validation of RNAi by real time PCR

Abstract

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