Abstract
Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.
Originalsprog | Engelsk |
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Tidsskrift | Methods in Molecular Biology |
Vol/bind | 703 |
Sider (fra-til) | 205-17 |
Antal sider | 13 |
ISSN | 1064-3745 |
DOI | |
Status | Udgivet - 2011 |