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Urinary excretion of total isothiocyanates from cruciferous vegetables shows high dose-response relationship and may be a useful biomarker for isothiocyanate exposure

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@article{64b110f418ef43bf9aca71c22c28008a,
title = "Urinary excretion of total isothiocyanates from cruciferous vegetables shows high dose-response relationship and may be a useful biomarker for isothiocyanate exposure",
abstract = "BACKGROUND: Isothiocyanates (ITCs), hydrolysis products from glucosinolates, are a family of biologically active compounds originating from cruciferous vegetables. Many ITCs are assumed to have cancer preventive effects and to further evaluate these potential health effects, reliable biomarkers of ITC exposure are needed.AIM OF THE STUDY: In this study we investigated the ability of urinary ITC excretion to reflect a low or high daily intake of cruciferous vegetables.METHODS: The design was a controlled human crossover study (n = 6). Subjects consumed a self-restricted glucosinolate-free diet 48 h before the study-day where a basic diet supplemented with 80 or 350 g of mixed cruciferous vegetables was consumed. All urine was collected in intervals during the 48 h period after ingestion of the cruciferous vegetables. Total ITC in the cruciferous mixture and total ITC and their metabolites in urine was quantified as the cyclocondensation product of 1,2-bezenedithiol by high performance liquid chromatography.RESULTS: The total urinary excretion of ITCs correlated significantly with the two doses of ITC from diets with high or low cruciferous content (r (s )= 0.90, P < 0.01). The fraction of urinary ITC excreted was 69.02 +/- 11.57{\%} and 74.53 +/- 8.39{\%} of the amounts ingested for 80 and 350 g cruciferous vegetables, respectively.CONCLUSION: The results in this study indicate that the urinary excretion of ITCs, measured by use of the cyclocondesation reaction, is a useful and precise tool that may be used as a biomarker of ITC exposure in population based studies.",
keywords = "Adult, Anticarcinogenic Agents, Area Under Curve, Biological Markers, Brassicaceae, Chromatography, High Pressure Liquid, Cross-Over Studies, Diet, Dose-Response Relationship, Drug, Female, Humans, Isothiocyanates, Male, Metabolic Clearance Rate, Neoplasms, Urinalysis",
author = "Mette Kristensen and Krogholm, {Kirstine S} and Hanne Frederiksen and B{\"u}gel, {Susanne H} and Rasmussen, {Salka E}",
year = "2007",
month = "10",
doi = "10.1007/s00394-007-0676-5",
language = "English",
volume = "46",
pages = "377--82",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "Dr. Dietrich/Steinkopff Verlag",
number = "7",

}

RIS

TY - JOUR

T1 - Urinary excretion of total isothiocyanates from cruciferous vegetables shows high dose-response relationship and may be a useful biomarker for isothiocyanate exposure

AU - Kristensen, Mette

AU - Krogholm, Kirstine S

AU - Frederiksen, Hanne

AU - Bügel, Susanne H

AU - Rasmussen, Salka E

PY - 2007/10

Y1 - 2007/10

N2 - BACKGROUND: Isothiocyanates (ITCs), hydrolysis products from glucosinolates, are a family of biologically active compounds originating from cruciferous vegetables. Many ITCs are assumed to have cancer preventive effects and to further evaluate these potential health effects, reliable biomarkers of ITC exposure are needed.AIM OF THE STUDY: In this study we investigated the ability of urinary ITC excretion to reflect a low or high daily intake of cruciferous vegetables.METHODS: The design was a controlled human crossover study (n = 6). Subjects consumed a self-restricted glucosinolate-free diet 48 h before the study-day where a basic diet supplemented with 80 or 350 g of mixed cruciferous vegetables was consumed. All urine was collected in intervals during the 48 h period after ingestion of the cruciferous vegetables. Total ITC in the cruciferous mixture and total ITC and their metabolites in urine was quantified as the cyclocondensation product of 1,2-bezenedithiol by high performance liquid chromatography.RESULTS: The total urinary excretion of ITCs correlated significantly with the two doses of ITC from diets with high or low cruciferous content (r (s )= 0.90, P < 0.01). The fraction of urinary ITC excreted was 69.02 +/- 11.57% and 74.53 +/- 8.39% of the amounts ingested for 80 and 350 g cruciferous vegetables, respectively.CONCLUSION: The results in this study indicate that the urinary excretion of ITCs, measured by use of the cyclocondesation reaction, is a useful and precise tool that may be used as a biomarker of ITC exposure in population based studies.

AB - BACKGROUND: Isothiocyanates (ITCs), hydrolysis products from glucosinolates, are a family of biologically active compounds originating from cruciferous vegetables. Many ITCs are assumed to have cancer preventive effects and to further evaluate these potential health effects, reliable biomarkers of ITC exposure are needed.AIM OF THE STUDY: In this study we investigated the ability of urinary ITC excretion to reflect a low or high daily intake of cruciferous vegetables.METHODS: The design was a controlled human crossover study (n = 6). Subjects consumed a self-restricted glucosinolate-free diet 48 h before the study-day where a basic diet supplemented with 80 or 350 g of mixed cruciferous vegetables was consumed. All urine was collected in intervals during the 48 h period after ingestion of the cruciferous vegetables. Total ITC in the cruciferous mixture and total ITC and their metabolites in urine was quantified as the cyclocondensation product of 1,2-bezenedithiol by high performance liquid chromatography.RESULTS: The total urinary excretion of ITCs correlated significantly with the two doses of ITC from diets with high or low cruciferous content (r (s )= 0.90, P < 0.01). The fraction of urinary ITC excreted was 69.02 +/- 11.57% and 74.53 +/- 8.39% of the amounts ingested for 80 and 350 g cruciferous vegetables, respectively.CONCLUSION: The results in this study indicate that the urinary excretion of ITCs, measured by use of the cyclocondesation reaction, is a useful and precise tool that may be used as a biomarker of ITC exposure in population based studies.

KW - Adult

KW - Anticarcinogenic Agents

KW - Area Under Curve

KW - Biological Markers

KW - Brassicaceae

KW - Chromatography, High Pressure Liquid

KW - Cross-Over Studies

KW - Diet

KW - Dose-Response Relationship, Drug

KW - Female

KW - Humans

KW - Isothiocyanates

KW - Male

KW - Metabolic Clearance Rate

KW - Neoplasms

KW - Urinalysis

U2 - 10.1007/s00394-007-0676-5

DO - 10.1007/s00394-007-0676-5

M3 - Journal article

VL - 46

SP - 377

EP - 382

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 7

ER -

ID: 45288409