Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery

Eva Martha Madsen Svarrer; Henrik Ørbæk Andersen; Morten Helvind; Maartje C J Slagman; Gerjan Navis; Robin P F Dullaart; Björn Dahlbäck; Lars Bo Nielsen

doi:10.2217/bmm.15.105

Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery

Eva Martha Madsen Svarrer, Henrik Ørbæk Andersen, Morten Helvind, Maartje C J Slagman, Gerjan Navis, Robin P F Dullaart, Björn Dahlbäck, Lars Bo Nielsen

Afdeling for Klinisk Biokemi DIA

9 Citationer (Scopus)

Abstract

AIM: To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI).

MATERIALS & METHODS: Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was characterized by gel filtration chromatography and western blotting.

RESULTS: ApoM was excreted into the urine 0-4 h postoperatively as the full-length apoM in particles smaller than plasma HDL. At 0 h, U-apoM predicted AKI with an area under the receiver-operating characteristics curve of 0.70 (p < 0.018). Sensitivity was 0.71 and specificity was 0.68 at a cutoff level at 1.45 nmol/l.

CONCLUSION: ApoM is excreted in the urine of children after cardiac surgery. Its potential as biomarker of AKI deserves exploration.

Originalsprog	Engelsk
Tidsskrift	Biomarkers in Medicine
Vol/bind	10
Udgave nummer	1
Sider (fra-til)	81-93
Antal sider	13
ISSN	1752-0363
DOI	https://doi.org/10.2217/bmm.15.105
Status	Udgivet - 2016

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10.2217/bmm.15.105

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@article{31bb1274f2604708a4098a94a68555a9,

title = "Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery",

abstract = "AIM: To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI).MATERIALS & METHODS: Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was characterized by gel filtration chromatography and western blotting.RESULTS: ApoM was excreted into the urine 0-4 h postoperatively as the full-length apoM in particles smaller than plasma HDL. At 0 h, U-apoM predicted AKI with an area under the receiver-operating characteristics curve of 0.70 (p < 0.018). Sensitivity was 0.71 and specificity was 0.68 at a cutoff level at 1.45 nmol/l.CONCLUSION: ApoM is excreted in the urine of children after cardiac surgery. Its potential as biomarker of AKI deserves exploration.",

keywords = "Acute Kidney Injury, Acute-Phase Proteins, Apolipoproteins, Biomarkers, Cardiac Surgical Procedures, Child, Child, Preschool, Cohort Studies, Female, Humans, Infant, Infant, Newborn, Lipocalins, Male, Proto-Oncogene Proteins, Clinical Trial, Journal Article",

author = "Svarrer, {Eva Martha Madsen} and Andersen, {Henrik {\O}rb{\ae}k} and Morten Helvind and Slagman, {Maartje C J} and Gerjan Navis and Dullaart, {Robin P F} and Bj{\"o}rn Dahlb{\"a}ck and Nielsen, {Lars Bo}",

year = "2016",

doi = "10.2217/bmm.15.105",

language = "English",

volume = "10",

pages = "81--93",

journal = "Biomarkers in Medicine",

issn = "1752-0363",

publisher = "BioMed Central Ltd",

number = "1",

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TY - JOUR

T1 - Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery

AU - Svarrer, Eva Martha Madsen

AU - Andersen, Henrik Ørbæk

AU - Helvind, Morten

AU - Slagman, Maartje C J

AU - Navis, Gerjan

AU - Dullaart, Robin P F

AU - Dahlbäck, Björn

AU - Nielsen, Lars Bo

PY - 2016

Y1 - 2016

N2 - AIM: To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI).MATERIALS & METHODS: Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was characterized by gel filtration chromatography and western blotting.RESULTS: ApoM was excreted into the urine 0-4 h postoperatively as the full-length apoM in particles smaller than plasma HDL. At 0 h, U-apoM predicted AKI with an area under the receiver-operating characteristics curve of 0.70 (p < 0.018). Sensitivity was 0.71 and specificity was 0.68 at a cutoff level at 1.45 nmol/l.CONCLUSION: ApoM is excreted in the urine of children after cardiac surgery. Its potential as biomarker of AKI deserves exploration.

AB - AIM: To investigate whether apoM is excreted in urine of children undergoing heart surgery and the potential of apoM as early biomarker of acute kidney injury (AKI).MATERIALS & METHODS: Urine was collected in children undergoing heart surgery. ApoM was measured with ELISA. U-apoM was characterized by gel filtration chromatography and western blotting.RESULTS: ApoM was excreted into the urine 0-4 h postoperatively as the full-length apoM in particles smaller than plasma HDL. At 0 h, U-apoM predicted AKI with an area under the receiver-operating characteristics curve of 0.70 (p < 0.018). Sensitivity was 0.71 and specificity was 0.68 at a cutoff level at 1.45 nmol/l.CONCLUSION: ApoM is excreted in the urine of children after cardiac surgery. Its potential as biomarker of AKI deserves exploration.

KW - Acute Kidney Injury

KW - Acute-Phase Proteins

KW - Apolipoproteins

KW - Biomarkers

KW - Cardiac Surgical Procedures

KW - Child

KW - Child, Preschool

KW - Cohort Studies

KW - Female

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Lipocalins

KW - Male

KW - Proto-Oncogene Proteins

KW - Clinical Trial

KW - Journal Article

U2 - 10.2217/bmm.15.105

DO - 10.2217/bmm.15.105

M3 - Journal article

C2 - 26642098

SN - 1752-0363

VL - 10

SP - 81

EP - 93

JO - Biomarkers in Medicine

JF - Biomarkers in Medicine

IS - 1

ER -

Urinary apolipoprotein M as a biomarker of acute kidney injury in children undergoing heart surgery

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