Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

Alexandra Y C Kruse; Lasse Kvich; Steffen Eickhardt; Lars H Omland; Thomas Bjarnsholt; Claus Moser

doi:10.1128/JCM.03404-14

Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

Alexandra Y C Kruse
, Lasse Kvich
, Steffen Eickhardt
, Lars H Omland
, Thomas Bjarnsholt
, Claus Moser

Afdeling for Klinisk Mikrobiologi DIA

4 Citationer (Scopus)

Abstract

The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	53
Udgave nummer	6
Sider (fra-til)	1983-5
Antal sider	3
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.03404-14
Status	Udgivet - jun. 2015

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10.1128/JCM.03404-14

Citationsformater

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title = "Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing",

abstract = "The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.",

author = "Kruse, \{Alexandra Y C\} and Lasse Kvich and Steffen Eickhardt and Omland, \{Lars H\} and Thomas Bjarnsholt and Claus Moser",

year = "2015",

month = jun,

doi = "10.1128/JCM.03404-14",

language = "English",

volume = "53",

pages = "1983--5",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

AU - Kruse, Alexandra Y C

AU - Kvich, Lasse

AU - Eickhardt, Steffen

AU - Omland, Lars H

AU - Bjarnsholt, Thomas

AU - Moser, Claus

PY - 2015/6

Y1 - 2015/6

N2 - The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

AB - The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

U2 - 10.1128/JCM.03404-14

DO - 10.1128/JCM.03404-14

M3 - Journal article

C2 - 25854484

SN - 0095-1137

VL - 53

SP - 1983

EP - 1985

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

Abstract

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