Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Transfusion Strategies are Associated with Epigenetic Changes Following Blunt Trauma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Absences of Endothelial Microvesicle Changes in the Presence of the Endotheliopathy of Trauma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Acute Lung Injury in Critically Ill Patients: Actin-Scavenger Gelsolin Signals Prolonged Respiratory Failure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Lactate is a Prognostic Factor in Patients Admitted with Suspected ST-Elevation Myocardial Infarction

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Plasma Resuscitation Improved Survival in a Cecal Ligation and Puncture Rat Model of Sepsis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Ketamine as a Rapid Sequence Induction Agent in the Trauma Population: A Systematic Review

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The effect of fluid resuscitation strategy on monocyte and T-cell surface markers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Tubastatin A prevents hemorrhage-induced endothelial barrier dysfunction

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Self-evaluated competence in trauma reception

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

INTRODUCTION: Epigenetics has been identified in multiple diseases. The effect of transfusion strategy on epigenetics is unknown. We hypothesized that expression of epigenetic regulating genes would be associated with resuscitation strategy following blunt trauma.

METHODS: Retrospective study using the inflammation in host response to injury (glue grant) dataset. Volume transfused over 24 h of packed red blood cells (PRBC), fresh frozen plasma (FFP), platelets (PLT) as well as crystalloids was extracted along with leucocyte microarray data of genes with known epigenetic modulating activity from day 1 after injury.Principal component analysis (PCA) was used to identify principal components (PC) within the transcriptomic dataset. Multiple regression associated these with volume of blood products and crystalloids while controlling for confounders. Genes co-expressed with genes central genes in the identified PCs were furthermore subjected to pathway analysis using the reactome database.

RESULTS: PCA identified seven components. PRBC and crystalloid volumes were positively associated with PC1, implicating histone acetylation (HAT1), DNA and histone methylation (KDM6B, SET1DB) and histone phosphorylation (RPS6KA5). Conversely, PLT volume was negatively associated with PC1,5 and 6, implicating DNA methylation (DNMT1) as well as histone acetylation (HAT1) and phosphorylation (RPSKA3).FFP was negatively associated with PC3, implicating histone methylation (SETD1B) and phosphorylation (RPS6KA5).Co-expression network analysis identified downstream pathways relevant to inflammation and the innate immune response, including Toll-like receptor, interleukin and mitogen activated protein kinase activation.

CONCLUSIONS: Resuscitation strategy was associated with epigenetic transcriptomic alterations, especially for PRBC and PLT transfusions. Downstream effects may include pertubations of pathways involved in immune signaling and cellular survival.

OriginalsprogEngelsk
TidsskriftShock
Vol/bind50
Udgave nummer1
Sider (fra-til)24-30
Antal sider7
ISSN1073-2322
DOI
StatusUdgivet - jul. 2018

ID: 56536170