Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

B K Müller; P Kabos; B Belhage; T Neumann; S B Kater

Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

B K Müller
, P Kabos
, B Belhage
, T Neumann
, S B Kater

Anæstesiafdelingen BFH

5 Citationer (Scopus)

Abstract

Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

Originalsprog	Engelsk
Tidsskrift	European Journal of Cell Biology
Vol/bind	69
Udgave nummer	4
Sider (fra-til)	360-7
Antal sider	8
ISSN	0171-9335
Status	Udgivet - 1 apr. 1996

Citationsformater

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title = "Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells",

abstract = "Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.",

author = "M{\"u}ller, \{B K\} and P Kabos and B Belhage and T Neumann and Kater, \{S B\}",

year = "1996",

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volume = "69",

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TY - JOUR

T1 - Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

AU - Müller, B K

AU - Kabos, P

AU - Belhage, B

AU - Neumann, T

AU - Kater, S B

PY - 1996/4/1

Y1 - 1996/4/1

N2 - Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

AB - Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

M3 - Journal article

C2 - 8741218

SN - 0171-9335

VL - 69

SP - 360

EP - 367

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

IS - 4

ER -

Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

Abstract

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