Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

B K Müller; P Kabos; B Belhage; T Neumann; S B Kater

Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

B K Müller, P Kabos, B Belhage, T Neumann, S B Kater

Anæstesiafd. Z

5 Citationer (Scopus)

Abstrakt

Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

Originalsprog	Engelsk
Tidsskrift	European Journal of Cell Biology
Vol/bind	69
Udgave nummer	4
Sider (fra-til)	360-7
Antal sider	8
ISSN	0171-9335
Status	Udgivet - 1 apr. 1996

Citationsformater

@article{25729097e1bd49e3914cc49c14cd6dce,

title = "Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells",

abstract = "Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.",

author = "M{\"u}ller, {B K} and P Kabos and B Belhage and T Neumann and Kater, {S B}",

year = "1996",

month = apr,

day = "1",

language = "English",

volume = "69",

pages = "360--7",

journal = "European Journal of Cell Biology",

issn = "0171-9335",

publisher = "Urban und Fischer Verlag",

number = "4",

}

TY - JOUR

T1 - Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

AU - Müller, B K

AU - Kabos, P

AU - Belhage, B

AU - Neumann, T

AU - Kater, S B

PY - 1996/4/1

Y1 - 1996/4/1

N2 - Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

AB - Indirect evidence supports a protective role of some EF-hand calcium-binding proteins against calcium-induced neurotoxicity. Little is known about how these proteins influence cytosolic calcium levels. After cloning the parvalbumin cDNA into an expression vector, teratocarcinoma cells (PCC7) were transfected. Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. The results show that parvalbumin-transfected PCC7 cells had much better calcium buffering capacity than control cells.

M3 - Journal article

C2 - 8741218

VL - 69

SP - 360

EP - 367

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 4

ER -

Transfected parvalbumin alters calcium homeostasis in teratocarcinoma PCC7 cells

Abstrakt

Fingeraftryk

Citationsformater