Harvard
Paul, F, Arkin, Y, Giladi, A, Jaitin, DA, Kenigsberg, E, Keren-Shaul, H, Winter, D, Lara-Astiaso, D, Gury, M, Weiner, A, David, E, Cohen, N
, Lauridsen, FKB, Haas, S, Schlitzer, A, Mildner, A, Ginhoux, F, Jung, S, Trumpp, A
, Porse, BT, Tanay, A & Amit, I 2015, '
Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors'
Cell, bind 163, nr. 7, s. 1663-77.
https://doi.org/10.1016/j.cell.2015.11.013
APA
Paul, F., Arkin, Y., Giladi, A., Jaitin, D. A., Kenigsberg, E., Keren-Shaul, H., ... Amit, I. (2015).
Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors.
Cell,
163(7), 1663-77.
https://doi.org/10.1016/j.cell.2015.11.013
CBE
Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H, Winter D, Lara-Astiaso D, Gury M, Weiner A, David E, Cohen N
, Lauridsen FKB, Haas S, Schlitzer A, Mildner A, Ginhoux F, Jung S, Trumpp A
, Porse BT, Tanay A, Amit I. 2015.
Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors.
Cell. 163(7):1663-77.
https://doi.org/10.1016/j.cell.2015.11.013
MLA
Vancouver
Author
Paul, Franziska ; Arkin, Ya'ara ; Giladi, Amir ; Jaitin, Diego Adhemar ; Kenigsberg, Ephraim ; Keren-Shaul, Hadas ; Winter, Deborah ; Lara-Astiaso, David ; Gury, Meital ; Weiner, Assaf ; David, Eyal ; Cohen, Nadav
; Lauridsen, Felicia Kathrine Bratt ; Haas, Simon ; Schlitzer, Andreas ; Mildner, Alexander ; Ginhoux, Florent ; Jung, Steffen ; Trumpp, Andreas
; Porse, Bo Torben ; Tanay, Amos ; Amit, Ido. /
Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors. I:
Cell. 2015 ; Bind 163, Nr. 7. s. 1663-77.
Bibtex
@article{fe3f9ed3085848e29ce2b096a56204fc,
title = "Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors",
abstract = "Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.",
author = "Franziska Paul and Ya'ara Arkin and Amir Giladi and Jaitin, {Diego Adhemar} and Ephraim Kenigsberg and Hadas Keren-Shaul and Deborah Winter and David Lara-Astiaso and Meital Gury and Assaf Weiner and Eyal David and Nadav Cohen and Lauridsen, {Felicia Kathrine Bratt} and Simon Haas and Andreas Schlitzer and Alexander Mildner and Florent Ginhoux and Steffen Jung and Andreas Trumpp and Porse, {Bo Torben} and Amos Tanay and Ido Amit",
note = "Copyright {\circledC} 2015 Elsevier Inc. All rights reserved.",
year = "2015",
month = "12",
day = "17",
doi = "10.1016/j.cell.2015.11.013",
language = "English",
volume = "163",
pages = "1663--77",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",
}
RIS
TY - JOUR
T1 - Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors
AU - Paul, Franziska
AU - Arkin, Ya'ara
AU - Giladi, Amir
AU - Jaitin, Diego Adhemar
AU - Kenigsberg, Ephraim
AU - Keren-Shaul, Hadas
AU - Winter, Deborah
AU - Lara-Astiaso, David
AU - Gury, Meital
AU - Weiner, Assaf
AU - David, Eyal
AU - Cohen, Nadav
AU - Lauridsen, Felicia Kathrine Bratt
AU - Haas, Simon
AU - Schlitzer, Andreas
AU - Mildner, Alexander
AU - Ginhoux, Florent
AU - Jung, Steffen
AU - Trumpp, Andreas
AU - Porse, Bo Torben
AU - Tanay, Amos
AU - Amit, Ido
N1 - Copyright © 2015 Elsevier Inc. All rights reserved.
PY - 2015/12/17
Y1 - 2015/12/17
N2 - Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.
AB - Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.
U2 - 10.1016/j.cell.2015.11.013
DO - 10.1016/j.cell.2015.11.013
M3 - Journal article
VL - 163
SP - 1663
EP - 1677
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -