Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors

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Region Hovedstaden - en del af Københavns Universitetshospital

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Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Paul, F, Arkin, Y, Giladi, A, Jaitin, DA, Kenigsberg, E, Keren-Shaul, H, Winter, D, Lara-Astiaso, D, Gury, M, Weiner, A, David, E, Cohen, N, Lauridsen, FKB, Haas, S, Schlitzer, A, Mildner, A, Ginhoux, F, Jung, S, Trumpp, A, Porse, BT, Tanay, A & Amit, I 2015, 'Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors' Cell, bind 163, nr. 7, s. 1663-77. https://doi.org/10.1016/j.cell.2015.11.013

APA

Paul, F., Arkin, Y., Giladi, A., Jaitin, D. A., Kenigsberg, E., Keren-Shaul, H., ... Amit, I. (2015). Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors. Cell, 163(7), 1663-77. https://doi.org/10.1016/j.cell.2015.11.013

CBE

Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H, Winter D, Lara-Astiaso D, Gury M, Weiner A, David E, Cohen N, Lauridsen FKB, Haas S, Schlitzer A, Mildner A, Ginhoux F, Jung S, Trumpp A, Porse BT, Tanay A, Amit I. 2015. Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors. Cell. 163(7):1663-77. https://doi.org/10.1016/j.cell.2015.11.013

MLA

Paul, Franziska o.a.. "Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors". Cell. 2015, 163(7). 1663-77. https://doi.org/10.1016/j.cell.2015.11.013

Vancouver

Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H o.a. Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors. Cell. 2015 dec 17;163(7):1663-77. https://doi.org/10.1016/j.cell.2015.11.013

Author

Paul, Franziska ; Arkin, Ya'ara ; Giladi, Amir ; Jaitin, Diego Adhemar ; Kenigsberg, Ephraim ; Keren-Shaul, Hadas ; Winter, Deborah ; Lara-Astiaso, David ; Gury, Meital ; Weiner, Assaf ; David, Eyal ; Cohen, Nadav ; Lauridsen, Felicia Kathrine Bratt ; Haas, Simon ; Schlitzer, Andreas ; Mildner, Alexander ; Ginhoux, Florent ; Jung, Steffen ; Trumpp, Andreas ; Porse, Bo Torben ; Tanay, Amos ; Amit, Ido. / Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors. I: Cell. 2015 ; Bind 163, Nr. 7. s. 1663-77.

Bibtex

@article{fe3f9ed3085848e29ce2b096a56204fc,

title = "Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors",

abstract = "Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.",

author = "Franziska Paul and Ya'ara Arkin and Amir Giladi and Jaitin, {Diego Adhemar} and Ephraim Kenigsberg and Hadas Keren-Shaul and Deborah Winter and David Lara-Astiaso and Meital Gury and Assaf Weiner and Eyal David and Nadav Cohen and Lauridsen, {Felicia Kathrine Bratt} and Simon Haas and Andreas Schlitzer and Alexander Mildner and Florent Ginhoux and Steffen Jung and Andreas Trumpp and Porse, {Bo Torben} and Amos Tanay and Ido Amit",

year = "2015",

month = "12",

day = "17",

doi = "10.1016/j.cell.2015.11.013",

language = "English",

volume = "163",

pages = "1663--77",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "7",

}

RIS

TY - JOUR

T1 - Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors

AU - Paul, Franziska

AU - Arkin, Ya'ara

AU - Giladi, Amir

AU - Jaitin, Diego Adhemar

AU - Kenigsberg, Ephraim

AU - Keren-Shaul, Hadas

AU - Winter, Deborah

AU - Lara-Astiaso, David

AU - Gury, Meital

AU - Weiner, Assaf

AU - David, Eyal

AU - Cohen, Nadav

AU - Lauridsen, Felicia Kathrine Bratt

AU - Haas, Simon

AU - Schlitzer, Andreas

AU - Mildner, Alexander

AU - Ginhoux, Florent

AU - Jung, Steffen

AU - Trumpp, Andreas

AU - Porse, Bo Torben

AU - Tanay, Amos

AU - Amit, Ido

PY - 2015/12/17

Y1 - 2015/12/17

N2 - Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.

AB - Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.

U2 - 10.1016/j.cell.2015.11.013

DO - 10.1016/j.cell.2015.11.013

M3 - Journal article

VL - 163

SP - 1663

EP - 1677

JO - Cell

JF - Cell

SN - 0092-8674

IS - 7

ER -

ID: 45854893