Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Low-Frequency and Rare-Coding Variation Contributes to Multiple Sclerosis Risk

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Genomic Dissection of Bipolar Disorder and Schizophrenia, Including 28 Subphenotypes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Drug-Driven Phenotypic Convergence Supports Rational Treatment Strategies of Chronic Infections

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. CGRP Antibodies as Prophylaxis in Migraine

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Mutant CEBPA directly drives the expression of the targetable tumor-promoting factor CD73 in AML

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Heterozygous loss of Srp72 in mice is not associated with major hematological phenotypes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. The splicing factor RBM25 controls MYC activity in acute myeloid leukemia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Leukemogenic nucleophosmin mutation disrupts the transcription factor hub regulating granulo-monocytic fates

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Franziska Paul
  • Ya'ara Arkin
  • Amir Giladi
  • Diego Adhemar Jaitin
  • Ephraim Kenigsberg
  • Hadas Keren-Shaul
  • Deborah Winter
  • David Lara-Astiaso
  • Meital Gury
  • Assaf Weiner
  • Eyal David
  • Nadav Cohen
  • Felicia Kathrine Bratt Lauridsen
  • Simon Haas
  • Andreas Schlitzer
  • Alexander Mildner
  • Florent Ginhoux
  • Steffen Jung
  • Andreas Trumpp
  • Bo Torben Porse
  • Amos Tanay
  • Ido Amit
Vis graf over relationer

Within the bone marrow, stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with in vivo differentiation potential or gene regulatory mechanisms. Here, we comprehensively map myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups, showing unexpected transcriptional priming toward seven differentiation fates but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting that the process is tightly regulated. Histone maps and knockout assays are consistent with early transcriptional priming, while traditional transplantation experiments suggest that in vivo priming may still allow for plasticity given strong perturbations. These data establish a reference model and general framework for studying hematopoiesis at single-cell resolution.

OriginalsprogEngelsk
TidsskriftCell
Vol/bind163
Udgave nummer7
Sider (fra-til)1663-77
ISSN0092-8674
DOI
StatusUdgivet - 17 dec. 2015

ID: 45854893