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Time and temperature dependent analytical stability of dry-collected Evalyn HPV self-sampling brush for cervical cancer screening

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Bibtex

@article{4d232107b6f1477796e168b92680fcf0,
title = "Time and temperature dependent analytical stability of dry-collected Evalyn HPV self-sampling brush for cervical cancer screening",
abstract = "As a new initiative, HPV self-sampling to non-attenders using the dry Evalyn self-sampling brush is offered in the Capital Region of Denmark. The use of a dry brush is largely uncharted territory in terms of analytical stability. In this study we aim to provide evidence on the analytical quality of dry HPV self-sampling brushes as a function of time and temperature. We assessed the analytical stability of dry stored Evalyn brushes at three different temperatures, (4°C, room temperature, 30°C) and five different storage time points; T=0 (baseline), 2, 4, 8, 16, and 32 weeks prior to HPV analysis using the BD Onclarity HPV assay. Mean Ct value of the Onclarity internal control was used as comparator of cellularity across time and temperatures, with no or only borderline statistical differences observed. HPV detection was stable throughout the five time points. In addition, analytically amplifiable DNA copy numbers and DNA fragmentation was assessed using the Agena iPLEX Exome QC assay, with no or only borderline statistical differences observed. In conclusion, the Evalyn brush is analytically stable with respect to human genomic material and HPV detection for up to 32 weeks at temperatures ranging from 4°C to 30°C.",
keywords = "Journal Article",
author = "Ejegod, {Ditte M{\o}ller} and Helle Pedersen and Alzua, {Garazi Pe{\~n}a} and Camilla Pedersen and Jesper Bonde",
note = "Copyright {\circledC} 2018 The Authors. Published by Elsevier B.V. All rights reserved.",
year = "2018",
month = "6",
doi = "10.1016/j.pvr.2018.04.005",
language = "English",
volume = "5",
pages = "192--200",
journal = "Papillomavirus Research",
issn = "2405-8521",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Time and temperature dependent analytical stability of dry-collected Evalyn HPV self-sampling brush for cervical cancer screening

AU - Ejegod, Ditte Møller

AU - Pedersen, Helle

AU - Alzua, Garazi Peña

AU - Pedersen, Camilla

AU - Bonde, Jesper

N1 - Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

PY - 2018/6

Y1 - 2018/6

N2 - As a new initiative, HPV self-sampling to non-attenders using the dry Evalyn self-sampling brush is offered in the Capital Region of Denmark. The use of a dry brush is largely uncharted territory in terms of analytical stability. In this study we aim to provide evidence on the analytical quality of dry HPV self-sampling brushes as a function of time and temperature. We assessed the analytical stability of dry stored Evalyn brushes at three different temperatures, (4°C, room temperature, 30°C) and five different storage time points; T=0 (baseline), 2, 4, 8, 16, and 32 weeks prior to HPV analysis using the BD Onclarity HPV assay. Mean Ct value of the Onclarity internal control was used as comparator of cellularity across time and temperatures, with no or only borderline statistical differences observed. HPV detection was stable throughout the five time points. In addition, analytically amplifiable DNA copy numbers and DNA fragmentation was assessed using the Agena iPLEX Exome QC assay, with no or only borderline statistical differences observed. In conclusion, the Evalyn brush is analytically stable with respect to human genomic material and HPV detection for up to 32 weeks at temperatures ranging from 4°C to 30°C.

AB - As a new initiative, HPV self-sampling to non-attenders using the dry Evalyn self-sampling brush is offered in the Capital Region of Denmark. The use of a dry brush is largely uncharted territory in terms of analytical stability. In this study we aim to provide evidence on the analytical quality of dry HPV self-sampling brushes as a function of time and temperature. We assessed the analytical stability of dry stored Evalyn brushes at three different temperatures, (4°C, room temperature, 30°C) and five different storage time points; T=0 (baseline), 2, 4, 8, 16, and 32 weeks prior to HPV analysis using the BD Onclarity HPV assay. Mean Ct value of the Onclarity internal control was used as comparator of cellularity across time and temperatures, with no or only borderline statistical differences observed. HPV detection was stable throughout the five time points. In addition, analytically amplifiable DNA copy numbers and DNA fragmentation was assessed using the Agena iPLEX Exome QC assay, with no or only borderline statistical differences observed. In conclusion, the Evalyn brush is analytically stable with respect to human genomic material and HPV detection for up to 32 weeks at temperatures ranging from 4°C to 30°C.

KW - Journal Article

U2 - 10.1016/j.pvr.2018.04.005

DO - 10.1016/j.pvr.2018.04.005

M3 - Journal article

VL - 5

SP - 192

EP - 200

JO - Papillomavirus Research

JF - Papillomavirus Research

SN - 2405-8521

ER -

ID: 53715082