Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Dickkopf-3 links HSF1 and YAP/TAZ signalling to control aggressive behaviours in cancer-associated fibroblasts

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Shared heritability and functional enrichment across six solid cancers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Author Correction: The challenge of mapping the human connectome based on diffusion tractography

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Molecular profiling of tissue biopsies reveals unique signatures associated with streptococcal necrotizing soft tissue infections

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Joint sequencing of human and pathogen genomes reveals the genetics of pneumococcal meningitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Isolation of metastatic circulating tumor cells (CTCs) from cancer patients is of high value for disease monitoring and molecular characterization. Despite the development of many new CTC isolation platforms in the last decade, their isolation and detection has remained a challenge due to the lack of specific and sensitive markers. In this feasibility study, we present a method for CTC isolation based on the specific binding of the malaria rVAR2 protein to oncofetal chondroitin sulfate (ofCS). We show that rVAR2 efficiently captures CTCs from hepatic, lung, pancreatic, and prostate carcinoma patients with minimal contamination of peripheral blood mononuclear cells. Expression of ofCS is present on epithelial and mesenchymal cancer cells and is equally preserved during epithelial-mesenchymal transition of cancer cells. In 25 stage I-IV prostate cancer patient samples, CTC enumeration significantly correlates with disease stage. Lastly, rVAR2 targets a larger and more diverse population of CTCs compared to anti-EpCAM strategies.

OriginalsprogEngelsk
TidsskriftNature Communications
Vol/bind9
Udgave nummer1
Sider (fra-til)3279
ISSN2041-1723
DOI
StatusUdgivet - 16 aug. 2018

ID: 56450083