The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

L H Engelholm, B S Nielsen, S Netzel-Arnett, H Solberg, X D Chen, J M Lopez Garcia, C Lopez-Otin, M F Young, H Birkedal-Hansen, K Danø, L R Lund, N Behrendt, T H Bugge

    65 Citationer (Scopus)

    Abstract

    The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

    OriginalsprogEngelsk
    TidsskriftLaboratory investigation; a journal of technical methods and pathology
    Vol/bind81
    Udgave nummer10
    Sider (fra-til)1403-14
    Antal sider12
    ISSN0023-6837
    StatusUdgivet - okt. 2001

    Fingeraftryk

    Dyk ned i forskningsemnerne om 'The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis'. Sammen danner de et unikt fingeraftryk.

    Citationsformater