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The transcriptome of peripheral blood mononuclear cells in patients with clinical subtypes of late age-related macular degeneration

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Subhi, Yousif ; Nielsen, Marie Krogh ; Molbech, Christopher Rue ; Liisborg, Charlotte ; Søndergaard, Helle Bach ; Sellebjerg, Finn ; Sørensen, Torben Lykke. / The transcriptome of peripheral blood mononuclear cells in patients with clinical subtypes of late age-related macular degeneration. I: Immunity and Ageing. 2019 ; Bind 16. s. e20.

Bibtex

@article{c2339bbaf6b043e1ab1734b4fee79503,
title = "The transcriptome of peripheral blood mononuclear cells in patients with clinical subtypes of late age-related macular degeneration",
abstract = "Background: Peripheral blood mononuclear cells (PBMCs) are implicated in the pathogenesis of age-related macular degeneration (AMD). We here mapped the global gene transcriptome of PBMCs from patients with different clinical subtypes of late AMD.Results: We sampled fresh venous blood from patients with geographic atrophy (GA) secondary to AMD without choroidal neovascularizations (n = 19), patients with neovascular AMD without GA (n = 38), patients with polypoidal choroidal vasculopathy (PCV) (n = 19), and aged control individuals with healthy retinae (n = 20). We isolated PBMCs, extracted RNA, and used microarray to investigate gene expression. Volcano plots identified statistically significant differentially expressed genes (P < 0.05) at a high magnitude (≥30{\%} higher/lower) for GA (62 genes), neovascular AMD (41 genes), and PCV (41 genes). These clinical subtypes differed substantially across gene expression and the following pathways identified in enrichment analyses. In a subgroup analysis, we investigated presence vs. absence of subretinal fibrosis and found 826 differentially expressed genes (≥30{\%} higher/lower, P < 0.05) with relation to mRNA splicing, endothelial migration, and interleukin-1 signaling.Conclusions: We here map the global gene transcriptome of PBMCs related to clinical subtypes of late AMD and find evidence of subtype-specific immunological involvement. Our findings provide a transcriptomic insight into the systemic immunity associated with AMD.",
author = "Yousif Subhi and Nielsen, {Marie Krogh} and Molbech, {Christopher Rue} and Charlotte Liisborg and S{\o}ndergaard, {Helle Bach} and Finn Sellebjerg and S{\o}rensen, {Torben Lykke}",
year = "2019",
doi = "10.1186/s12979-019-0160-0",
language = "English",
volume = "16",
pages = "e20",
journal = "Immunity and Ageing",
issn = "1742-4933",
publisher = "BioMed Central Ltd",

}

RIS

TY - JOUR

T1 - The transcriptome of peripheral blood mononuclear cells in patients with clinical subtypes of late age-related macular degeneration

AU - Subhi, Yousif

AU - Nielsen, Marie Krogh

AU - Molbech, Christopher Rue

AU - Liisborg, Charlotte

AU - Søndergaard, Helle Bach

AU - Sellebjerg, Finn

AU - Sørensen, Torben Lykke

PY - 2019

Y1 - 2019

N2 - Background: Peripheral blood mononuclear cells (PBMCs) are implicated in the pathogenesis of age-related macular degeneration (AMD). We here mapped the global gene transcriptome of PBMCs from patients with different clinical subtypes of late AMD.Results: We sampled fresh venous blood from patients with geographic atrophy (GA) secondary to AMD without choroidal neovascularizations (n = 19), patients with neovascular AMD without GA (n = 38), patients with polypoidal choroidal vasculopathy (PCV) (n = 19), and aged control individuals with healthy retinae (n = 20). We isolated PBMCs, extracted RNA, and used microarray to investigate gene expression. Volcano plots identified statistically significant differentially expressed genes (P < 0.05) at a high magnitude (≥30% higher/lower) for GA (62 genes), neovascular AMD (41 genes), and PCV (41 genes). These clinical subtypes differed substantially across gene expression and the following pathways identified in enrichment analyses. In a subgroup analysis, we investigated presence vs. absence of subretinal fibrosis and found 826 differentially expressed genes (≥30% higher/lower, P < 0.05) with relation to mRNA splicing, endothelial migration, and interleukin-1 signaling.Conclusions: We here map the global gene transcriptome of PBMCs related to clinical subtypes of late AMD and find evidence of subtype-specific immunological involvement. Our findings provide a transcriptomic insight into the systemic immunity associated with AMD.

AB - Background: Peripheral blood mononuclear cells (PBMCs) are implicated in the pathogenesis of age-related macular degeneration (AMD). We here mapped the global gene transcriptome of PBMCs from patients with different clinical subtypes of late AMD.Results: We sampled fresh venous blood from patients with geographic atrophy (GA) secondary to AMD without choroidal neovascularizations (n = 19), patients with neovascular AMD without GA (n = 38), patients with polypoidal choroidal vasculopathy (PCV) (n = 19), and aged control individuals with healthy retinae (n = 20). We isolated PBMCs, extracted RNA, and used microarray to investigate gene expression. Volcano plots identified statistically significant differentially expressed genes (P < 0.05) at a high magnitude (≥30% higher/lower) for GA (62 genes), neovascular AMD (41 genes), and PCV (41 genes). These clinical subtypes differed substantially across gene expression and the following pathways identified in enrichment analyses. In a subgroup analysis, we investigated presence vs. absence of subretinal fibrosis and found 826 differentially expressed genes (≥30% higher/lower, P < 0.05) with relation to mRNA splicing, endothelial migration, and interleukin-1 signaling.Conclusions: We here map the global gene transcriptome of PBMCs related to clinical subtypes of late AMD and find evidence of subtype-specific immunological involvement. Our findings provide a transcriptomic insight into the systemic immunity associated with AMD.

U2 - 10.1186/s12979-019-0160-0

DO - 10.1186/s12979-019-0160-0

M3 - Journal article

VL - 16

SP - e20

JO - Immunity and Ageing

JF - Immunity and Ageing

SN - 1742-4933

ER -

ID: 59099282