Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

The transcriptome of peripheral blood mononuclear cells in patients with clinical subtypes of late age-related macular degeneration

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Healthy lifestyles reduce suPAR and mortality in a Danish general population study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. MAIT cell subtypes in multiple sclerosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Early Intrathecal T Helper 17.1 Cell Activity in Huntington Disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Intoxicationer og ernæringsdeficit

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiUndervisning

  4. Klinisk Neurologi og Neurokirurgi

    Publikation: Bog/antologi/afhandling/rapportBogUndervisning

Vis graf over relationer

Background: Peripheral blood mononuclear cells (PBMCs) are implicated in the pathogenesis of age-related macular degeneration (AMD). We here mapped the global gene transcriptome of PBMCs from patients with different clinical subtypes of late AMD.

Results: We sampled fresh venous blood from patients with geographic atrophy (GA) secondary to AMD without choroidal neovascularizations (n = 19), patients with neovascular AMD without GA (n = 38), patients with polypoidal choroidal vasculopathy (PCV) (n = 19), and aged control individuals with healthy retinae (n = 20). We isolated PBMCs, extracted RNA, and used microarray to investigate gene expression. Volcano plots identified statistically significant differentially expressed genes (P < 0.05) at a high magnitude (≥30% higher/lower) for GA (62 genes), neovascular AMD (41 genes), and PCV (41 genes). These clinical subtypes differed substantially across gene expression and the following pathways identified in enrichment analyses. In a subgroup analysis, we investigated presence vs. absence of subretinal fibrosis and found 826 differentially expressed genes (≥30% higher/lower, P < 0.05) with relation to mRNA splicing, endothelial migration, and interleukin-1 signaling.

Conclusions: We here map the global gene transcriptome of PBMCs related to clinical subtypes of late AMD and find evidence of subtype-specific immunological involvement. Our findings provide a transcriptomic insight into the systemic immunity associated with AMD.

OriginalsprogEngelsk
TidsskriftImmunity and Ageing
Vol/bind16
Sider (fra-til)e20
ISSN1742-4933
DOI
StatusUdgivet - 2019

ID: 59099282