Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Small-molecule inhibitors of Ataxia Telangiectasia and Rad3 related kinase (ATR) sensitize lymphoma cells to UVA radiation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Dose dependent sun protective effect of topical melatonin: A randomized, placebo-controlled, double-blind study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. MicroRNAs and potential target interactions in psoriasis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Subdermal penetration of topically applied clobetasol propionate in ointment base through intact human skin

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Degree of employment, sick leave, and costs following notification of occupational contact dermatitis-A register-based study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Clinical manifestations and impact on daily life of allergy to polyethylene glycol (PEG) in ten patients

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Contact dermatitis caused by glucose sensors, insulin pumps, and tapes: Results from a 5-year period

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Patch test results with the European baseline series and additions thereof in the ESSCA network, 2015-2018

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: Skin biopsies represent a gold standard in skin immunology and pathology but can cause pain and induce scarring. Non-invasive techniques will facilitate study recruitment of e.g. patients with paediatric atopic dermatitis (AD), hand eczema or facial dermatitis.

OBJECTIVE: By RNA sequencing, we examined whether the stratum corneum transcriptome in AD skin can be assessed by tape stripping, as compared to the epidermal transcriptome of AD in skin biopsies. To make the procedure clinically relevant tape strips were stored and shipped at room temperature for up to 3 days.

METHODS: Nine adult Caucasian AD patients and three healthy volunteers were included. Tape samples were collected from non-lesional and lesional skin. Biopsies were collected from lesional skin and were split into epidermis and dermis. Total RNA was extracted, and shotgun sequencing was performed.

RESULTS: Shotgun sequencing could be performed on skin cells obtained from two consecutive tape strips which had been stored and shipped at room temperature for up to three days. The most prominent differences between the tape strip and biopsy derived transcriptome were due to structural genes, while established molecular markers of AD, including CCL17, CCL22, IL17A and S100A7-S100A9, were also identified in tape strip samples. Furthermore, the tape strip derived transcriptome showed promise in also analysing the skin microbiome.

CONCLUSION: Our study shows that the stratum corneum (SC) transcriptome of AD can be assessed by tape stripping the skin, supporting that this method may be central in future skin biomarker research. NCBI GEO data accession: GSE160501.

OriginalsprogEngelsk
TidsskriftJournal of Dermatological Science
Vol/bind101
Udgave nummer1
Sider (fra-til)14-21
Antal sider8
ISSN0923-1811
DOI
StatusUdgivet - jan. 2021

Bibliografisk note

Copyright © 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.

ID: 61947730