Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Distinct Autoimmune Anti-α-Synuclein Antibody Patterns in Multiple System Atrophy and Parkinson’s Disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Authors' response: Association between IBD and Parkinson's disease: seek and you shall find?

    Publikation: Bidrag til tidsskriftKommentar/debatForskning

  3. Misfolded SOD1 inclusions in patients with mutations in C9orf72 and other ALS/FTD-associated genes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Neurostereologic Lesion Volumes and Spreading Depolarizations in Severe Traumatic Brain Injury Patients: A Pilot Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
Stereological methods are designed to describe quantitative parameters without making assumptions about size, shape, orientation and distribution of cells or structures. These methods have been revolutionary for quantitative analysis of the mammalian brain, in which volumetric cell populations are too high to count manually, and stereology is now the technique of choice whenever estimates of three-dimensional quantities need to be extracted from measurements on two-dimensional sections. All stereological methods are in principle unbiased; however, they rely on proper knowledge about the structure of interest and the characteristics of the tissue. Stereology is based on Systematic Uniformly Random Sampling (SURS), with adjustment of sampling to the most efficient level in respect to precision, providing reliable, quantitative information about the whole structure of interest. Here we present the optical fractionator in conjunction with BrdU immunohistochemistry to estimate the production and survival of newly-formed neurons in the granule cell layer (including the sub-granular zone) of the rat hippocampus following electroconvulsive stimulation, which is among the most potent stimulators of neurogenesis. The optical fractionator technique is designed to provide estimates of the total number of cells from thick sections sampled from the full structure. Thick sections provide the opportunity to observe cells in their full 3-D extent and thus, allow for easy and robust cell classification based on morphological criteria. When correctly implemented, the sensitivity and efficiency of the optical fractionator provides accurate estimates with a fixed and predetermined precision.
OriginalsprogEngelsk
TidsskriftJournal of Visualized Experiments
Udgave nummer125
ISSN1940-087X
DOI
StatusUdgivet - 9 jul. 2017

ID: 52335070