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The occurrence of biofilm in an equine experimental wound model of healing by secondary intention

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Jørgensen, E ; Bay, L ; Bjarnsholt, T ; Bundgaard, L ; Sørensen, M A ; Jacobsen, S. / The occurrence of biofilm in an equine experimental wound model of healing by secondary intention. I: Veterinary Microbiology. 2017 ; Bind 204. s. 90-95.

Bibtex

@article{f63e2ce9a1b946439aa260c2de7278d1,
title = "The occurrence of biofilm in an equine experimental wound model of healing by secondary intention",
abstract = "In humans, biofilm is a well-known cause of delayed healing and low-grade inflammation of chronic wounds. In horses, biofilm formation in wounds has been studied to a very limited degree. The objective of this study was thus to investigate the occurrence of biofilm in equine experimental wounds healing by secondary intention. Tissue biopsies from non-contaminated, experimental excisional shoulder and limb wounds were obtained on day 1-2, day 7-10 and day 14-15 post-wounding. Limb wounds were either un-bandaged or bandaged to induce exuberant granulation tissue (EGT) formation and thereby impaired healing. Presence of biofilm in tissue biopsies was assessed by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) and confocal laser scanning microscopy (CLSM). Bandaged limb wounds developed EGT and displayed delayed healing, while shoulder and un-bandaged limb wounds healed normally. Biofilm was detected in limb wounds only. At day 14-15 biofilm was significantly more prevalent in bandaged limb wounds than in un-bandaged limb wounds (P=0.003). Further, bandaged limb wounds had a statistically significant increase in biofilm burden from day 7-10 to day 14-15 (P=0.009). The finding that biofilm was most prevalent in bandaged limb wounds with EGT formation suggests that biofilm may be linked to delayed wound healing in horses, as has been observed in humans. The inability to clear bacteria could be related to hypoxia and low-grade inflammation in the EGT, but the interaction between biofilm forming bacteria and wound healing in horses needs further elucidation.",
keywords = "Animals, Bacteria, Bandages, Biofilms, Horses, Male, Wound Healing, Journal Article",
author = "E J{\o}rgensen and L Bay and T Bjarnsholt and L Bundgaard and S{\o}rensen, {M A} and S Jacobsen",
note = "Copyright {\textcopyright} 2017 Elsevier B.V. All rights reserved.",
year = "2017",
month = may,
doi = "10.1016/j.vetmic.2017.03.011",
language = "English",
volume = "204",
pages = "90--95",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - The occurrence of biofilm in an equine experimental wound model of healing by secondary intention

AU - Jørgensen, E

AU - Bay, L

AU - Bjarnsholt, T

AU - Bundgaard, L

AU - Sørensen, M A

AU - Jacobsen, S

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2017/5

Y1 - 2017/5

N2 - In humans, biofilm is a well-known cause of delayed healing and low-grade inflammation of chronic wounds. In horses, biofilm formation in wounds has been studied to a very limited degree. The objective of this study was thus to investigate the occurrence of biofilm in equine experimental wounds healing by secondary intention. Tissue biopsies from non-contaminated, experimental excisional shoulder and limb wounds were obtained on day 1-2, day 7-10 and day 14-15 post-wounding. Limb wounds were either un-bandaged or bandaged to induce exuberant granulation tissue (EGT) formation and thereby impaired healing. Presence of biofilm in tissue biopsies was assessed by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) and confocal laser scanning microscopy (CLSM). Bandaged limb wounds developed EGT and displayed delayed healing, while shoulder and un-bandaged limb wounds healed normally. Biofilm was detected in limb wounds only. At day 14-15 biofilm was significantly more prevalent in bandaged limb wounds than in un-bandaged limb wounds (P=0.003). Further, bandaged limb wounds had a statistically significant increase in biofilm burden from day 7-10 to day 14-15 (P=0.009). The finding that biofilm was most prevalent in bandaged limb wounds with EGT formation suggests that biofilm may be linked to delayed wound healing in horses, as has been observed in humans. The inability to clear bacteria could be related to hypoxia and low-grade inflammation in the EGT, but the interaction between biofilm forming bacteria and wound healing in horses needs further elucidation.

AB - In humans, biofilm is a well-known cause of delayed healing and low-grade inflammation of chronic wounds. In horses, biofilm formation in wounds has been studied to a very limited degree. The objective of this study was thus to investigate the occurrence of biofilm in equine experimental wounds healing by secondary intention. Tissue biopsies from non-contaminated, experimental excisional shoulder and limb wounds were obtained on day 1-2, day 7-10 and day 14-15 post-wounding. Limb wounds were either un-bandaged or bandaged to induce exuberant granulation tissue (EGT) formation and thereby impaired healing. Presence of biofilm in tissue biopsies was assessed by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) and confocal laser scanning microscopy (CLSM). Bandaged limb wounds developed EGT and displayed delayed healing, while shoulder and un-bandaged limb wounds healed normally. Biofilm was detected in limb wounds only. At day 14-15 biofilm was significantly more prevalent in bandaged limb wounds than in un-bandaged limb wounds (P=0.003). Further, bandaged limb wounds had a statistically significant increase in biofilm burden from day 7-10 to day 14-15 (P=0.009). The finding that biofilm was most prevalent in bandaged limb wounds with EGT formation suggests that biofilm may be linked to delayed wound healing in horses, as has been observed in humans. The inability to clear bacteria could be related to hypoxia and low-grade inflammation in the EGT, but the interaction between biofilm forming bacteria and wound healing in horses needs further elucidation.

KW - Animals

KW - Bacteria

KW - Bandages

KW - Biofilms

KW - Horses

KW - Male

KW - Wound Healing

KW - Journal Article

U2 - 10.1016/j.vetmic.2017.03.011

DO - 10.1016/j.vetmic.2017.03.011

M3 - Journal article

C2 - 28532812

VL - 204

SP - 90

EP - 95

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -

ID: 52705914