The need for transparency and good practices in the qPCR literature

Stephen A Bustin; Vladimir Benes; Jeremy Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory Shipley; Carl T Wittwer; Peter Schjerling; Philip J Day; Mónica Abreu; Begoña Aguado; Jean-François Beaulieu; Anneleen Beckers; Sara Bogaert; John A Browne; Fernando Carrasco-Ramiro; Liesbeth Ceelen; Kate Ciborowski; Pieter Cornillie; Stephanie Coulon; Ann Cuypers; Sara De Brouwer; Leentje De Ceuninck; Jurgen De Craene; Hélène De Naeyer; Ward De Spiegelaere; Kato Deckers; Annelies Dheedene; Kaat Durinck; Margarida Ferreira-Teixeira; Annelies Fieuw; Jack M Gallup; Sandra Gonzalo-Flores; Karen Goossens; Femke Heindryckx; Elizabeth Herring; Hans Hoenicka; Laura Icardi; Rolf Jaggi; Farzad Javad; Michael Karampelias; Frederick Kibenge; Molly Kibenge; Candy Kumps; Irina Lambertz; Tim Lammens; Amelia Markey; Peter Messiaen; Evelien Mets; Sofia Morais; Alberto Mudarra-Rubio; Justine Nakiwala; Hilde Nelis; Pal A Olsvik; Claudina Pérez-Novo; Michelle Plusquin; Tony Remans; Ali Rihani; Paulo Rodrigues-Santos; Pieter Rondou; Rebecca Sanders; Katharina Schmidt-Bleek; Kerstin Skovgaard; Karen Smeets; Laura Tabera; Stefan Toegel; Tim Van Acker; Wim Van den Broeck; Joni Van der Meulen; Mireille Van Gele; Gert Van Peer; Mario Van Poucke; Nadine Van Roy; Sarah Vergult; Joris Wauman; Marina Tshuikina-Wiklander; Erik Willems; Sara Zaccara; Fjoralba Zeka; Jo Vandesompele

doi:10.1038/nmeth.2697

The need for transparency and good practices in the qPCR literature

Stephen A Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, Carl T Wittwer, Peter Schjerling, Philip J Day, Mónica Abreu, Begoña Aguado, Jean-François Beaulieu, Anneleen Beckers, Sara Bogaert, John A Browne, Fernando Carrasco-RamiroLiesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Hélène De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michael Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A Olsvik, Claudina Pérez-Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, Jo Vandesompele

Ortopædkirurgisk Afd. M

233 Citationer (Scopus)

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Originalsprog	Engelsk
Tidsskrift	Nature Methods
Vol/bind	10
Udgave nummer	11
Sider (fra-til)	1063-7
Antal sider	5
ISSN	1548-7091
DOI	https://doi.org/10.1038/nmeth.2697
Status	Udgivet - nov. 2013

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10.1038/nmeth.2697

Citationsformater

Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G., Wittwer, C. T., Schjerling, P., Day, P. J., Abreu, M., Aguado, B., Beaulieu, J.-F., Beckers, A., Bogaert, S., Browne, J. A., ... Vandesompele, J. (2013). The need for transparency and good practices in the qPCR literature. Nature Methods, 10(11), 1063-7. https://doi.org/10.1038/nmeth.2697

Bustin, SA, Benes, V, Garson, J, Hellemans, J, Huggett, J, Kubista, M, Mueller, R, Nolan, T, Pfaffl, MW, Shipley, G, Wittwer, CT, Schjerling, P, Day, PJ, Abreu, M, Aguado, B, Beaulieu, J-F, Beckers, A, Bogaert, S, Browne, JA, Carrasco-Ramiro, F, Ceelen, L, Ciborowski, K, Cornillie, P, Coulon, S, Cuypers, A, De Brouwer, S, De Ceuninck, L, De Craene, J, De Naeyer, H, De Spiegelaere, W, Deckers, K, Dheedene, A, Durinck, K, Ferreira-Teixeira, M, Fieuw, A, Gallup, JM, Gonzalo-Flores, S, Goossens, K, Heindryckx, F, Herring, E, Hoenicka, H, Icardi, L, Jaggi, R, Javad, F, Karampelias, M, Kibenge, F, Kibenge, M, Kumps, C, Lambertz, I, Lammens, T, Markey, A, Messiaen, P, Mets, E, Morais, S, Mudarra-Rubio, A, Nakiwala, J, Nelis, H, Olsvik, PA, Pérez-Novo, C, Plusquin, M, Remans, T, Rihani, A, Rodrigues-Santos, P, Rondou, P, Sanders, R, Schmidt-Bleek, K, Skovgaard, K, Smeets, K, Tabera, L, Toegel, S, Van Acker, T, Van den Broeck, W, Van der Meulen, J, Van Gele, M, Van Peer, G, Van Poucke, M, Van Roy, N, Vergult, S, Wauman, J, Tshuikina-Wiklander, M, Willems, E, Zaccara, S, Zeka, F & Vandesompele, J 2013, 'The need for transparency and good practices in the qPCR literature', Nature Methods, bind 10, nr. 11, s. 1063-7. https://doi.org/10.1038/nmeth.2697

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AU - Benes, Vladimir

AU - Garson, Jeremy

AU - Hellemans, Jan

AU - Huggett, Jim

AU - Kubista, Mikael

AU - Mueller, Reinhold

AU - Nolan, Tania

AU - Pfaffl, Michael W

AU - Shipley, Gregory

AU - Wittwer, Carl T

AU - Schjerling, Peter

AU - Day, Philip J

AU - Abreu, Mónica

AU - Aguado, Begoña

AU - Beaulieu, Jean-François

AU - Beckers, Anneleen

AU - Bogaert, Sara

AU - Browne, John A

AU - Carrasco-Ramiro, Fernando

AU - Ceelen, Liesbeth

AU - Ciborowski, Kate

AU - Cornillie, Pieter

AU - Coulon, Stephanie

AU - Cuypers, Ann

AU - De Brouwer, Sara

AU - De Ceuninck, Leentje

AU - De Craene, Jurgen

AU - De Naeyer, Hélène

AU - De Spiegelaere, Ward

AU - Deckers, Kato

AU - Dheedene, Annelies

AU - Durinck, Kaat

AU - Ferreira-Teixeira, Margarida

AU - Fieuw, Annelies

AU - Gallup, Jack M

AU - Gonzalo-Flores, Sandra

AU - Goossens, Karen

AU - Heindryckx, Femke

AU - Herring, Elizabeth

AU - Hoenicka, Hans

AU - Icardi, Laura

AU - Jaggi, Rolf

AU - Javad, Farzad

AU - Karampelias, Michael

AU - Kibenge, Frederick

AU - Kibenge, Molly

AU - Kumps, Candy

AU - Lambertz, Irina

AU - Lammens, Tim

AU - Markey, Amelia

AU - Messiaen, Peter

AU - Mets, Evelien

AU - Morais, Sofia

AU - Mudarra-Rubio, Alberto

AU - Nakiwala, Justine

AU - Nelis, Hilde

AU - Olsvik, Pal A

AU - Pérez-Novo, Claudina

AU - Plusquin, Michelle

AU - Remans, Tony

AU - Rihani, Ali

AU - Rodrigues-Santos, Paulo

AU - Rondou, Pieter

AU - Sanders, Rebecca

AU - Schmidt-Bleek, Katharina

AU - Skovgaard, Kerstin

AU - Smeets, Karen

AU - Tabera, Laura

AU - Toegel, Stefan

AU - Van Acker, Tim

AU - Van den Broeck, Wim

AU - Van der Meulen, Joni

AU - Van Gele, Mireille

AU - Van Peer, Gert

AU - Van Poucke, Mario

AU - Van Roy, Nadine

AU - Vergult, Sarah

AU - Wauman, Joris

AU - Tshuikina-Wiklander, Marina

AU - Willems, Erik

AU - Zaccara, Sara

AU - Zeka, Fjoralba

AU - Vandesompele, Jo

PY - 2013/11

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N2 - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

AB - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

KW - Data Collection

KW - Information Services

KW - Polymerase Chain Reaction

U2 - 10.1038/nmeth.2697

DO - 10.1038/nmeth.2697

M3 - Journal article

C2 - 24173381

SN - 1548-7091

VL - 10

SP - 1063

EP - 1067

JO - Nature Methods

JF - Nature Methods

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ER -

The need for transparency and good practices in the qPCR literature

Abstract

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