The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

Benedicte Jørgenrud; Sirkku Jäntti; Ismo Mattila; Päivi Pöhö; Kjersti S Rønningen; Hannele Yki-Järvinen; Matej Orešič; Tuulia Hyötyläinen

doi:10.4155/bio.15.16

The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

Benedicte Jørgenrud, Sirkku Jäntti, Ismo Mattila, Päivi Pöhö, Kjersti S Rønningen, Hannele Yki-Järvinen, Matej Orešič, Tuulia Hyötyläinen

25 Citationer (Scopus)

Abstrakt

AIM: Blood serum and plasma have intrinsic differences in their composition and the preprocessing, such as clotting temperature in serum, and storage at room temperature may have further effect on metabolite concentrations.

METHODS: The influence of sampling preprocessing on the metabolic profiles in serum and different types of plasma was investigated using liquid chromatography and comprehensive 2D gas chromatography coupled to a mass spectrometer.

RESULTS: The profiles of polar metabolites were significantly dependent on the type of the sample, while lipid profiles were similar in serum and different types of plasma. Extended storage of plasma at room temperature resulted in degradation of lipids already after 1 day. Serum clotting at room temperature generally resulted in higher metabolite concentration compared with serum clotting on ice.

Originalsprog	Engelsk
Tidsskrift	Bioanalysis
Vol/bind	7
Udgave nummer	8
Sider (fra-til)	991-1006
Antal sider	16
ISSN	1757-6180
DOI	https://doi.org/10.4155/bio.15.16
Status	Udgivet - 2015
Udgivet eksternt	Ja

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10.4155/bio.15.16

Citationsformater

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title = "The influence of sample collection methodology and sample preprocessing on the blood metabolic profile",

abstract = "AIM: Blood serum and plasma have intrinsic differences in their composition and the preprocessing, such as clotting temperature in serum, and storage at room temperature may have further effect on metabolite concentrations.METHODS: The influence of sampling preprocessing on the metabolic profiles in serum and different types of plasma was investigated using liquid chromatography and comprehensive 2D gas chromatography coupled to a mass spectrometer.RESULTS: The profiles of polar metabolites were significantly dependent on the type of the sample, while lipid profiles were similar in serum and different types of plasma. Extended storage of plasma at room temperature resulted in degradation of lipids already after 1 day. Serum clotting at room temperature generally resulted in higher metabolite concentration compared with serum clotting on ice.",

keywords = "Adult, Chromatography, Liquid/methods, Female, Gas Chromatography-Mass Spectrometry/methods, Humans, Lipids/analysis, Male, Metabolome, Metabolomics/methods, Middle Aged, Plasma/chemistry, Serum/chemistry, Specimen Handling/methods",

author = "Benedicte J{\o}rgenrud and Sirkku J{\"a}ntti and Ismo Mattila and P{\"a}ivi P{\"o}h{\"o} and R{\o}nningen, {Kjersti S} and Hannele Yki-J{\"a}rvinen and Matej Ore{\v s}i{\v c} and Tuulia Hy{\"o}tyl{\"a}inen",

year = "2015",

doi = "10.4155/bio.15.16",

language = "English",

volume = "7",

pages = "991--1006",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Future Science Ltd",

number = "8",

}

TY - JOUR

T1 - The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

AU - Jørgenrud, Benedicte

AU - Jäntti, Sirkku

AU - Mattila, Ismo

AU - Pöhö, Päivi

AU - Rønningen, Kjersti S

AU - Yki-Järvinen, Hannele

AU - Orešič, Matej

AU - Hyötyläinen, Tuulia

PY - 2015

Y1 - 2015

N2 - AIM: Blood serum and plasma have intrinsic differences in their composition and the preprocessing, such as clotting temperature in serum, and storage at room temperature may have further effect on metabolite concentrations.METHODS: The influence of sampling preprocessing on the metabolic profiles in serum and different types of plasma was investigated using liquid chromatography and comprehensive 2D gas chromatography coupled to a mass spectrometer.RESULTS: The profiles of polar metabolites were significantly dependent on the type of the sample, while lipid profiles were similar in serum and different types of plasma. Extended storage of plasma at room temperature resulted in degradation of lipids already after 1 day. Serum clotting at room temperature generally resulted in higher metabolite concentration compared with serum clotting on ice.

AB - AIM: Blood serum and plasma have intrinsic differences in their composition and the preprocessing, such as clotting temperature in serum, and storage at room temperature may have further effect on metabolite concentrations.METHODS: The influence of sampling preprocessing on the metabolic profiles in serum and different types of plasma was investigated using liquid chromatography and comprehensive 2D gas chromatography coupled to a mass spectrometer.RESULTS: The profiles of polar metabolites were significantly dependent on the type of the sample, while lipid profiles were similar in serum and different types of plasma. Extended storage of plasma at room temperature resulted in degradation of lipids already after 1 day. Serum clotting at room temperature generally resulted in higher metabolite concentration compared with serum clotting on ice.

KW - Adult

KW - Chromatography, Liquid/methods

KW - Female

KW - Gas Chromatography-Mass Spectrometry/methods

KW - Humans

KW - Lipids/analysis

KW - Male

KW - Metabolome

KW - Metabolomics/methods

KW - Middle Aged

KW - Plasma/chemistry

KW - Serum/chemistry

KW - Specimen Handling/methods

U2 - 10.4155/bio.15.16

DO - 10.4155/bio.15.16

M3 - Journal article

C2 - 25966010

VL - 7

SP - 991

EP - 1006

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 8

ER -

The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

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