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Region Hovedstaden - en del af Københavns Universitetshospital
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The influence of direct and indirect fibroblast cell contact on human myogenic cell behavior and gene expression in vitro

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  1. Mechanical properties, physiological behavior, and function of aponeurosis and tendon

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  2. Plasma Free Fatty Acid concentration is closely tied to whole-body peak fat oxidation rate during repeated exercise

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  3. Effects of active commuting and leisure-time exercise on appetite in individuals with overweight and obesity

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  4. Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle

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  1. Maintenance of muscle strength following a one-year resistance training program in older adults

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  2. The influence of prolonged strength training upon muscle and fat in healthy and chronically diseased older adults

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  3. The effect of 4 months exercise training on systemic biomarkers of cartilage and bone turnover in hip osteoarthritis patients

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  4. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

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  5. The influence of fibrillin-1 and physical activity upon tendon tissue morphology and mechanical properties in mice

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Vis graf over relationer

Underpinning skeletal muscle plasticity is the interplay between many cell types, of which fibroblasts are emerging as potent players, both negatively in the development of fibrosis but also positively in stimulating muscle repair through enhancing myogenesis. The mechanisms behind this interaction however remain unknown. To investigate this, waste hamstring muscle tissue was obtained from eight healthy young men undergoing reconstructive anterior cruciate ligament surgery and primary myoblasts and fibroblasts were isolated. Myoblasts were cultured alone or with fibroblasts, either in direct or indirect contact (separated by an insert with a permeable membrane). The myogenesis parameters proliferation, differentiation, and fusion were determined from immunostained cells, while, in replicate samples, gene expression levels of GAPDH, Ki67, Pax7, MyoD, myogenin, myomaker, MHC-Iβ, TCF7L2, COL1A1, and p16 were determined by RT-PCR. We found only trends for an influence of skeletal muscle fibroblasts on myogenic cell proliferation and differentiation. While greater mRNA levels of GAPDH, Pax7, MyoD, myogenin, and MHC-Iβ were observed in myogenic cells in indirect contact with fibroblasts (insert) when compared with cells cultured alone, a similar effect of an empty insert was also observed. In conclusion we find very little influence of skeletal muscle fibroblasts on myoblasts derived from the same tissue, although it cannot be excluded that a different outcome would be seen under less optimal myogenic growth conditions.NEW & NOTEWORTHY Using passage one primary myoblasts and fibroblasts isolated from human skeletal muscle, we found only a trend for an effect of skeletal muscle fibroblasts on myogenic cell proliferation and differentiation. This is contrary to previous reports and raises the possibility that fibroblasts of different tissue origins exert distinct roles.

OriginalsprogEngelsk
TidsskriftJournal of Applied Physiology
Vol/bind127
Udgave nummer2
Sider (fra-til)342-355
Antal sider14
ISSN0161-7567
DOI
StatusUdgivet - 1 aug. 2019

ID: 59361877