The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.

H V Clausen; L Grupe Larsen; A Jørgensen; M Bzorek

doi:10.1111/j.1600-0463.2007.apm_492.x

The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.

H V Clausen, L Grupe Larsen, A Jørgensen, M Bzorek

Gynækologisk Obstetrisk Afdeling AHH

4 Citationer (Scopus)

Abstract

The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups.
Udgivelsesdato: 2007-Jan

Originalsprog	Engelsk
Tidsskrift	APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Vol/bind	115
Udgave nummer	1
Sider (fra-til)	22-9
Antal sider	7
ISSN	0903-4641
DOI	https://doi.org/10.1111/j.1600-0463.2007.apm_492.x
Status	Udgivet - 2007

Adgang til dokumentet

10.1111/j.1600-0463.2007.apm_492.x

Citationsformater

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title = "The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.",

abstract = "The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups. Udgivelsesdato: 2007-Jan",

keywords = "Adult, Endothelin-1, Endothelium, Vascular, Female, Humans, Immunohistochemistry, Nitric Oxide Synthase, Placenta, Pregnancy, Pregnancy Complications, Smoking",

author = "Clausen, {H V} and Larsen, {L Grupe} and A J{\o}rgensen and M Bzorek",

year = "2007",

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T1 - The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.

AU - Clausen, H V

AU - Larsen, L Grupe

AU - Jørgensen, A

AU - Bzorek, M

PY - 2007

Y1 - 2007

N2 - The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups. Udgivelsesdato: 2007-Jan

AB - The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups. Udgivelsesdato: 2007-Jan

KW - Adult

KW - Endothelin-1

KW - Endothelium, Vascular

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Nitric Oxide Synthase

KW - Placenta

KW - Pregnancy

KW - Pregnancy Complications

KW - Smoking

U2 - 10.1111/j.1600-0463.2007.apm_492.x

DO - 10.1111/j.1600-0463.2007.apm_492.x

M3 - Journal article

C2 - 17223848

SN - 0903-4641

VL - 115

SP - 22

EP - 29

JO - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

IS - 1

ER -