Abstrakt
The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups.
Udgivelsesdato: 2007-Jan
Udgivelsesdato: 2007-Jan
Originalsprog | Engelsk |
---|---|
Tidsskrift | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica |
Vol/bind | 115 |
Udgave nummer | 1 |
Sider (fra-til) | 22-9 |
Antal sider | 7 |
ISSN | 0903-4641 |
DOI | |
Status | Udgivet - 2007 |