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The host response to bacterial bone infection involves a local upregulation of several acute phase proteins

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Harvard

Lüthje, FL, Blirup-Plum, SA, Møller, NS, Heegaard, PMH, Jensen, HE, Kirketerp-Møller, K, Gottlieb, H, Skovgaard, K & Jensen, LK 2020, 'The host response to bacterial bone infection involves a local upregulation of several acute phase proteins', Immunobiology, bind 225, nr. 3, 151914, s. 151914. https://doi.org/10.1016/j.imbio.2020.151914

APA

Lüthje, F. L., Blirup-Plum, S. A., Møller, N. S., Heegaard, P. M. H., Jensen, H. E., Kirketerp-Møller, K., Gottlieb, H., Skovgaard, K., & Jensen, L. K. (2020). The host response to bacterial bone infection involves a local upregulation of several acute phase proteins. Immunobiology, 225(3), 151914. [151914]. https://doi.org/10.1016/j.imbio.2020.151914

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MLA

Vancouver

Author

Lüthje, Freja Lea ; Blirup-Plum, Sophie Amalie ; Møller, Nadia Sara ; Heegaard, Peter M H ; Jensen, Henrik Elvang ; Kirketerp-Møller, Klaus ; Gottlieb, Hans ; Skovgaard, Kerstin ; Jensen, Louise Kruse. / The host response to bacterial bone infection involves a local upregulation of several acute phase proteins. I: Immunobiology. 2020 ; Bind 225, Nr. 3. s. 151914.

Bibtex

@article{0066d8a9f9334bfa84a158e5000f8605,
title = "The host response to bacterial bone infection involves a local upregulation of several acute phase proteins",
abstract = "Bone infections often become chronic and can be difficult to diagnose. In the present study, the osseous gene expression of several acute phase proteins (APPs) during osteomyelitis was investigated in a porcine model of implant associated osteomyelitis (IAO) (sampled 5, 10 and 15 days after infection) and in slaughter pigs with spontaneous hematogenous osteomyelitis, and compared to gene expression in liver tissue. Furthermore, immunohistochemical (IHC) staining of the APP complement component C3 (C3) was performed on the porcine osteomyelitis lesions together with material from human patients with chronic osteomyelitis. In the porcine bone samples a local upregulation of the expression of several APP genes, including serum amyloid A (SAA) and C3, was observed during infection. In the liver, only C-reactive protein (CRP) and Inter-Alpha-Trypsin Inhibitor Heavy Chain 4 were significantly upregulated. Serum concentrations of CRP, SAA and haptoglobin were only upregulated at day 5 in infected animals of the IAO model. This indicates a limited systemic response to osteomyelitis. Similar numbers of positive IHC stained C3 leukocytes were found in human and porcine bone samples with chronic osteomyelitis, indicating a high transcriptional value of porcine models of osteomyelitis. The local upregulation of APPs could potentially be used for diagnosing osteomyelitis.",
keywords = "Acute phase proteins, Innate immunity, Osteomyelitis",
author = "L{\"u}thje, {Freja Lea} and Blirup-Plum, {Sophie Amalie} and M{\o}ller, {Nadia Sara} and Heegaard, {Peter M H} and Jensen, {Henrik Elvang} and Klaus Kirketerp-M{\o}ller and Hans Gottlieb and Kerstin Skovgaard and Jensen, {Louise Kruse}",
note = "Copyright {\textcopyright} 2020 Elsevier GmbH. All rights reserved.",
year = "2020",
month = may,
doi = "10.1016/j.imbio.2020.151914",
language = "English",
volume = "225",
pages = "151914",
journal = "Immunobiology",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag",
number = "3",

}

RIS

TY - JOUR

T1 - The host response to bacterial bone infection involves a local upregulation of several acute phase proteins

AU - Lüthje, Freja Lea

AU - Blirup-Plum, Sophie Amalie

AU - Møller, Nadia Sara

AU - Heegaard, Peter M H

AU - Jensen, Henrik Elvang

AU - Kirketerp-Møller, Klaus

AU - Gottlieb, Hans

AU - Skovgaard, Kerstin

AU - Jensen, Louise Kruse

N1 - Copyright © 2020 Elsevier GmbH. All rights reserved.

PY - 2020/5

Y1 - 2020/5

N2 - Bone infections often become chronic and can be difficult to diagnose. In the present study, the osseous gene expression of several acute phase proteins (APPs) during osteomyelitis was investigated in a porcine model of implant associated osteomyelitis (IAO) (sampled 5, 10 and 15 days after infection) and in slaughter pigs with spontaneous hematogenous osteomyelitis, and compared to gene expression in liver tissue. Furthermore, immunohistochemical (IHC) staining of the APP complement component C3 (C3) was performed on the porcine osteomyelitis lesions together with material from human patients with chronic osteomyelitis. In the porcine bone samples a local upregulation of the expression of several APP genes, including serum amyloid A (SAA) and C3, was observed during infection. In the liver, only C-reactive protein (CRP) and Inter-Alpha-Trypsin Inhibitor Heavy Chain 4 were significantly upregulated. Serum concentrations of CRP, SAA and haptoglobin were only upregulated at day 5 in infected animals of the IAO model. This indicates a limited systemic response to osteomyelitis. Similar numbers of positive IHC stained C3 leukocytes were found in human and porcine bone samples with chronic osteomyelitis, indicating a high transcriptional value of porcine models of osteomyelitis. The local upregulation of APPs could potentially be used for diagnosing osteomyelitis.

AB - Bone infections often become chronic and can be difficult to diagnose. In the present study, the osseous gene expression of several acute phase proteins (APPs) during osteomyelitis was investigated in a porcine model of implant associated osteomyelitis (IAO) (sampled 5, 10 and 15 days after infection) and in slaughter pigs with spontaneous hematogenous osteomyelitis, and compared to gene expression in liver tissue. Furthermore, immunohistochemical (IHC) staining of the APP complement component C3 (C3) was performed on the porcine osteomyelitis lesions together with material from human patients with chronic osteomyelitis. In the porcine bone samples a local upregulation of the expression of several APP genes, including serum amyloid A (SAA) and C3, was observed during infection. In the liver, only C-reactive protein (CRP) and Inter-Alpha-Trypsin Inhibitor Heavy Chain 4 were significantly upregulated. Serum concentrations of CRP, SAA and haptoglobin were only upregulated at day 5 in infected animals of the IAO model. This indicates a limited systemic response to osteomyelitis. Similar numbers of positive IHC stained C3 leukocytes were found in human and porcine bone samples with chronic osteomyelitis, indicating a high transcriptional value of porcine models of osteomyelitis. The local upregulation of APPs could potentially be used for diagnosing osteomyelitis.

KW - Acute phase proteins

KW - Innate immunity

KW - Osteomyelitis

UR - http://www.scopus.com/inward/record.url?scp=85079857417&partnerID=8YFLogxK

U2 - 10.1016/j.imbio.2020.151914

DO - 10.1016/j.imbio.2020.151914

M3 - Journal article

C2 - 32098686

VL - 225

SP - 151914

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 3

M1 - 151914

ER -

ID: 61913856