The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells

N Odum, S Bregenholt, K W Eriksen, Gitte Skov-Madsen, L P Ryder, K Bendtzen, R J Van Neerven, Arne Svejgaard, P Garred

23 Citationer (Scopus)

Abstrakt

The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.
OriginalsprogEngelsk
TidsskriftTissue Antigens
Vol/bind54
Udgave nummer6
Sider (fra-til)572-7
Antal sider6
ISSN0001-2815
StatusUdgivet - 1 dec. 1999

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