The B chain, but not the A chain, of Ricinus communis activates human complement

G Hetland; T E Mollnes; P Garred

doi:10.1016/0304-3835(93)90208-q

The B chain, but not the A chain, of Ricinus communis activates human complement

G Hetland, T E Mollnes, P Garred

1 Citationer (Scopus)

Abstract

Complement activation properties of ricin holotoxin, its A or B chain were assessed in two enzyme immunoassays (EIA). One was specific for C3 activation products and the other detected the terminal SC5b-9 complement complex (TCC) and thus determined activation of the initial and terminal part of the complement pathways, respectively. Ricin and its A and B chains were incubated with normal human serum or EDTA-serum. It was found that ricin B chain activated both the initial and terminal complement pathways in a time- and concentration-dependent fashion, whereas the A chain and the holotoxin did not.

Originalsprog	Engelsk
Tidsskrift	Cancer Letters
Vol/bind	75
Udgave nummer	1
Sider (fra-til)	59-63
Antal sider	5
ISSN	0304-3835
DOI	https://doi.org/10.1016/0304-3835(93)90208-q
Status	Udgivet - 30 nov. 1993
Udgivet eksternt	Ja

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10.1016/0304-3835(93)90208-q

Citationsformater

@article{86d9a8e09c474a279701e90e5a3b6cf3,

title = "The B chain, but not the A chain, of Ricinus communis activates human complement",

abstract = "Complement activation properties of ricin holotoxin, its A or B chain were assessed in two enzyme immunoassays (EIA). One was specific for C3 activation products and the other detected the terminal SC5b-9 complement complex (TCC) and thus determined activation of the initial and terminal part of the complement pathways, respectively. Ricin and its A and B chains were incubated with normal human serum or EDTA-serum. It was found that ricin B chain activated both the initial and terminal complement pathways in a time- and concentration-dependent fashion, whereas the A chain and the holotoxin did not.",

keywords = "Complement Activation, Complement C3/metabolism, Complement C5/metabolism, Complement C5b, Dose-Response Relationship, Drug, Edetic Acid/pharmacology, Humans, Immunoenzyme Techniques, In Vitro Techniques, Ricin/pharmacology",

author = "G Hetland and Mollnes, {T E} and P Garred",

year = "1993",

month = nov,

day = "30",

doi = "10.1016/0304-3835(93)90208-q",

language = "English",

volume = "75",

pages = "59--63",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - The B chain, but not the A chain, of Ricinus communis activates human complement

AU - Hetland, G

AU - Mollnes, T E

AU - Garred, P

PY - 1993/11/30

Y1 - 1993/11/30

N2 - Complement activation properties of ricin holotoxin, its A or B chain were assessed in two enzyme immunoassays (EIA). One was specific for C3 activation products and the other detected the terminal SC5b-9 complement complex (TCC) and thus determined activation of the initial and terminal part of the complement pathways, respectively. Ricin and its A and B chains were incubated with normal human serum or EDTA-serum. It was found that ricin B chain activated both the initial and terminal complement pathways in a time- and concentration-dependent fashion, whereas the A chain and the holotoxin did not.

AB - Complement activation properties of ricin holotoxin, its A or B chain were assessed in two enzyme immunoassays (EIA). One was specific for C3 activation products and the other detected the terminal SC5b-9 complement complex (TCC) and thus determined activation of the initial and terminal part of the complement pathways, respectively. Ricin and its A and B chains were incubated with normal human serum or EDTA-serum. It was found that ricin B chain activated both the initial and terminal complement pathways in a time- and concentration-dependent fashion, whereas the A chain and the holotoxin did not.

KW - Complement Activation

KW - Complement C3/metabolism

KW - Complement C5/metabolism

KW - Complement C5b

KW - Dose-Response Relationship, Drug

KW - Edetic Acid/pharmacology

KW - Humans

KW - Immunoenzyme Techniques

KW - In Vitro Techniques

KW - Ricin/pharmacology

U2 - 10.1016/0304-3835(93)90208-q

DO - 10.1016/0304-3835(93)90208-q

M3 - Journal article

C2 - 8287382

SN - 0304-3835

VL - 75

SP - 59

EP - 63

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

The B chain, but not the A chain, of Ricinus communis activates human complement

Abstract

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