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Test and validation of methods to sample and detect human virus from environmental surfaces using norovirus as a model virus

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@article{96a78d38cfe04ae48d603641cff09ca5,
title = "Test and validation of methods to sample and detect human virus from environmental surfaces using norovirus as a model virus",
abstract = "BACKGROUND: Viruses cause a major proportion of human infections, especially gastroenteritis and respiratory infections in children and adults. Indirect transmission between humans via environmental surfaces may play a role in infections, but methods to investigate this have been sparse.AIM: To validate and test efficient and reliable procedures to detect multiple human pathogenic viruses on surfaces.METHODS: The study was divided into two parts. In Part A, six combinations of three different swabs (consisting of cotton, foamed cotton, or polyester head) and two different elution methods (direct lysis or immersion in alkaline glycine buffer before lysis) were tested for efficient recovery of human norovirus GII.7 and mengovirus from artificially contaminated surfaces. In Part B we determined the detection limit for norovirus GI.1 and GII.3 using the best procedure found in Part A linked with a commercial multiplex real-time quantitative polymerase chain reaction detection assay.FINDINGS: Combining the polyester swab with direct lysis allowed recovery down to 100 and 10 genome copies/cm(2) of norovirus GI.1 and GII.3, respectively. This procedure resulted in the significant highest recovery of both norovirus and mengovirus, whereas no differences in amplification efficiencies were observed between the different procedures.CONCLUSION: The results indicate that it is possible to detect low concentrations of virus on environmental surfaces. We therefore suggest that a polyester swab, followed by direct lysis, combined with a multiplex qPCR detection assay is an efficient screening tool that merits study of different respiratory and gastrointestinal viruses on environment surfaces.",
keywords = "Environmental Microbiology, Humans, Mengovirus, Norovirus, Specimen Handling, Virology, Viruses, Journal Article, Research Support, Non-U.S. Gov't, Validation Studies",
author = "T Ibfelt and T Frandsen and A Permin and Andersen, {L P} and Schultz, {A C}",
note = "Copyright {\circledC} 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.",
year = "2016",
month = "4",
doi = "10.1016/j.jhin.2016.01.003",
language = "English",
volume = "92",
pages = "378--84",
journal = "Journal of Hospital Infection",
issn = "0195-6701",
publisher = "W.B./Saunders Co. Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Test and validation of methods to sample and detect human virus from environmental surfaces using norovirus as a model virus

AU - Ibfelt, T

AU - Frandsen, T

AU - Permin, A

AU - Andersen, L P

AU - Schultz, A C

N1 - Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

PY - 2016/4

Y1 - 2016/4

N2 - BACKGROUND: Viruses cause a major proportion of human infections, especially gastroenteritis and respiratory infections in children and adults. Indirect transmission between humans via environmental surfaces may play a role in infections, but methods to investigate this have been sparse.AIM: To validate and test efficient and reliable procedures to detect multiple human pathogenic viruses on surfaces.METHODS: The study was divided into two parts. In Part A, six combinations of three different swabs (consisting of cotton, foamed cotton, or polyester head) and two different elution methods (direct lysis or immersion in alkaline glycine buffer before lysis) were tested for efficient recovery of human norovirus GII.7 and mengovirus from artificially contaminated surfaces. In Part B we determined the detection limit for norovirus GI.1 and GII.3 using the best procedure found in Part A linked with a commercial multiplex real-time quantitative polymerase chain reaction detection assay.FINDINGS: Combining the polyester swab with direct lysis allowed recovery down to 100 and 10 genome copies/cm(2) of norovirus GI.1 and GII.3, respectively. This procedure resulted in the significant highest recovery of both norovirus and mengovirus, whereas no differences in amplification efficiencies were observed between the different procedures.CONCLUSION: The results indicate that it is possible to detect low concentrations of virus on environmental surfaces. We therefore suggest that a polyester swab, followed by direct lysis, combined with a multiplex qPCR detection assay is an efficient screening tool that merits study of different respiratory and gastrointestinal viruses on environment surfaces.

AB - BACKGROUND: Viruses cause a major proportion of human infections, especially gastroenteritis and respiratory infections in children and adults. Indirect transmission between humans via environmental surfaces may play a role in infections, but methods to investigate this have been sparse.AIM: To validate and test efficient and reliable procedures to detect multiple human pathogenic viruses on surfaces.METHODS: The study was divided into two parts. In Part A, six combinations of three different swabs (consisting of cotton, foamed cotton, or polyester head) and two different elution methods (direct lysis or immersion in alkaline glycine buffer before lysis) were tested for efficient recovery of human norovirus GII.7 and mengovirus from artificially contaminated surfaces. In Part B we determined the detection limit for norovirus GI.1 and GII.3 using the best procedure found in Part A linked with a commercial multiplex real-time quantitative polymerase chain reaction detection assay.FINDINGS: Combining the polyester swab with direct lysis allowed recovery down to 100 and 10 genome copies/cm(2) of norovirus GI.1 and GII.3, respectively. This procedure resulted in the significant highest recovery of both norovirus and mengovirus, whereas no differences in amplification efficiencies were observed between the different procedures.CONCLUSION: The results indicate that it is possible to detect low concentrations of virus on environmental surfaces. We therefore suggest that a polyester swab, followed by direct lysis, combined with a multiplex qPCR detection assay is an efficient screening tool that merits study of different respiratory and gastrointestinal viruses on environment surfaces.

KW - Environmental Microbiology

KW - Humans

KW - Mengovirus

KW - Norovirus

KW - Specimen Handling

KW - Virology

KW - Viruses

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Validation Studies

U2 - 10.1016/j.jhin.2016.01.003

DO - 10.1016/j.jhin.2016.01.003

M3 - Journal article

VL - 92

SP - 378

EP - 384

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

IS - 4

ER -

ID: 49928326