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Tendon collagen synthesis declines with immobilization in elderly humans: no effect of anti-inflammatory medication

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@article{56c3b99b86504d5f92ccf1c65a4c31c5,
title = "Tendon collagen synthesis declines with immobilization in elderly humans: no effect of anti-inflammatory medication",
abstract = "BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAID) are used as pain killers during periods of unloading caused by traumatic occurrences or diseases. However, it is unknown how the tendon protein turnover and mechanical properties responds to unloading and subsequent loading in elderly humans, and especially whether NSAIDs affect tendon adaptation during such periods. Thus, we studied the influence of NSAID upon the human patellar tendon protein synthesis and mechanical properties during immobilization and subsequent rehabilitating resistance training.METHODS: 19 men (60-80 yrs, range) were randomly assigned to NSAID (Ibuprofen 1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for two weeks and retrained for six weeks. Tendon collagen protein synthesis, expression of gene related to collagen turnover and remodeling, size, signal intensity (from magnetic resonance imaging), and mechanical properties were investigated.RESULTS: Tendon collagen synthesis decreased (p<0.001), whereas tendon size and mechanical properties were generally unchanged with immobilization, and NSAID treatment did not influence this. Matrix metalloproteinase-2 mRNA tended to increase (p<0.1) after immobilization in both groups, whereas scleraxis mRNA decreased with inactivity in the Plc group only (p<0.05).CONCLUSION: In elderly human tendons, collagen protein synthesis decreased after two weeks of immobilization, whereas tendon stiffness and modulus were only marginally reduced, and NSAID had no influence upon this. This indicates an importance of mechanical loading for maintenance of tendon collagen turnover. However, reduced collagen production induced by short-term unloading may only marginally affect tendon mechanical properties in elderly individuals.",
author = "Kasper Dideriksen and Boesen, {Anders Ploug} and S{\o}ren Reitelseder and Christian Couppe and Svensson, {Rene Br{\"u}ggebusch} and Peter Schjerling and Magnusson, {S Peter} and Lars Holm and Michael Kjaer",
note = "Copyright {\circledC} 2015, Journal of Applied Physiology.",
year = "2016",
month = "12",
day = "8",
doi = "10.1152/japplphysiol.00809.2015",
language = "English",
pages = "jap.00809.2015",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",

}

RIS

TY - JOUR

T1 - Tendon collagen synthesis declines with immobilization in elderly humans

T2 - no effect of anti-inflammatory medication

AU - Dideriksen, Kasper

AU - Boesen, Anders Ploug

AU - Reitelseder, Søren

AU - Couppe, Christian

AU - Svensson, Rene Brüggebusch

AU - Schjerling, Peter

AU - Magnusson, S Peter

AU - Holm, Lars

AU - Kjaer, Michael

N1 - Copyright © 2015, Journal of Applied Physiology.

PY - 2016/12/8

Y1 - 2016/12/8

N2 - BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAID) are used as pain killers during periods of unloading caused by traumatic occurrences or diseases. However, it is unknown how the tendon protein turnover and mechanical properties responds to unloading and subsequent loading in elderly humans, and especially whether NSAIDs affect tendon adaptation during such periods. Thus, we studied the influence of NSAID upon the human patellar tendon protein synthesis and mechanical properties during immobilization and subsequent rehabilitating resistance training.METHODS: 19 men (60-80 yrs, range) were randomly assigned to NSAID (Ibuprofen 1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for two weeks and retrained for six weeks. Tendon collagen protein synthesis, expression of gene related to collagen turnover and remodeling, size, signal intensity (from magnetic resonance imaging), and mechanical properties were investigated.RESULTS: Tendon collagen synthesis decreased (p<0.001), whereas tendon size and mechanical properties were generally unchanged with immobilization, and NSAID treatment did not influence this. Matrix metalloproteinase-2 mRNA tended to increase (p<0.1) after immobilization in both groups, whereas scleraxis mRNA decreased with inactivity in the Plc group only (p<0.05).CONCLUSION: In elderly human tendons, collagen protein synthesis decreased after two weeks of immobilization, whereas tendon stiffness and modulus were only marginally reduced, and NSAID had no influence upon this. This indicates an importance of mechanical loading for maintenance of tendon collagen turnover. However, reduced collagen production induced by short-term unloading may only marginally affect tendon mechanical properties in elderly individuals.

AB - BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAID) are used as pain killers during periods of unloading caused by traumatic occurrences or diseases. However, it is unknown how the tendon protein turnover and mechanical properties responds to unloading and subsequent loading in elderly humans, and especially whether NSAIDs affect tendon adaptation during such periods. Thus, we studied the influence of NSAID upon the human patellar tendon protein synthesis and mechanical properties during immobilization and subsequent rehabilitating resistance training.METHODS: 19 men (60-80 yrs, range) were randomly assigned to NSAID (Ibuprofen 1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for two weeks and retrained for six weeks. Tendon collagen protein synthesis, expression of gene related to collagen turnover and remodeling, size, signal intensity (from magnetic resonance imaging), and mechanical properties were investigated.RESULTS: Tendon collagen synthesis decreased (p<0.001), whereas tendon size and mechanical properties were generally unchanged with immobilization, and NSAID treatment did not influence this. Matrix metalloproteinase-2 mRNA tended to increase (p<0.1) after immobilization in both groups, whereas scleraxis mRNA decreased with inactivity in the Plc group only (p<0.05).CONCLUSION: In elderly human tendons, collagen protein synthesis decreased after two weeks of immobilization, whereas tendon stiffness and modulus were only marginally reduced, and NSAID had no influence upon this. This indicates an importance of mechanical loading for maintenance of tendon collagen turnover. However, reduced collagen production induced by short-term unloading may only marginally affect tendon mechanical properties in elderly individuals.

U2 - 10.1152/japplphysiol.00809.2015

DO - 10.1152/japplphysiol.00809.2015

M3 - Journal article

SP - jap.00809.2015

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

ER -

ID: 49585666