Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

TCF7L2 Expression Is Regulated by Cell Differentiation and Overfeeding in Human Adipose Tissue

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Epigenome- and Transcriptome-wide Changes in Muscle Stem Cells from Low Birth Weight Men

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Variations in serum 25-hydroxyvitamin D during acute pancreatitis: an exploratory longitudinal study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The age-specific incidence of type 2 diabetes in Danish elderly adults – the role of size at birth

    Publikation: KonferencebidragKonferenceabstrakt til konferenceForskningpeer review

  2. TET2 mutations are associated with hypermethylation at key regulatory enhancers in normal and malignant hematopoiesis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The role of vitamin C in epigenetic cancer therapy

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  4. VPS39-deficiency observed in type 2 diabetes impairs muscle stem cell differentiation via altered autophagy and epigenetics

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Aim: The TCF7L2 gene variant rs7903146 has the largest effect on type 2 diabetes risk reported in genome-wide association studies, however its role in adipose tissue development and function is unknown. We investigate the association between gene variant rs7903146 and metabolic parameters and examine in vitro and ex vivo gene expression of TCF7L2 in human adipose tissue and progenitor cells from two independent populations of young healthy men with increased risk of type 2 diabetes due to low birth weight (LBW). Design: Adipose tissue biopsies were excised from 40 healthy young men with low and normal birth weights (NBW) after a control and 5-day high-fat overfeeding diet. In another cohort including 13 LBW and 13 NBW men, adipocyte progenitor cells were isolated and cultivated. Transcriptome-wide expression was performed on RNA extracted from biopsies or cell cultures. Results: Diet-induced peripheral insulin resistance is more pronounced in carriers of the T-risk allele rs7903146, whereas no association with hepatic insulin resistance was shown. TCF7L2 expression increased during adipogenesis in isolated preadipocytes from both LBW and NBW men (p < 0.001) and correlated positively with markers of progenitor cell proliferation and maturation capacity. In the mature adipose tissue, LBW men had lower expression of TCF7L2 compared to NBW men at baseline (p = 0.03) and TCF7L2 expression was suppressed by short-term overfeeding in NBW men (p = 0.005). Conclusions: The results suggest a regulation of TCF7L2 expression during adipogenesis and in mature adipose tissue upon overfeeding, and further that young men exposed to an adverse intrauterine environment have reduced mature adipose tissue TCF7L2 expression.

OriginalsprogEngelsk
TidsskriftEndocrine Research
Vol/bind44
Udgave nummer3
Sider (fra-til)110-116
Antal sider7
ISSN0743-5800
DOI
StatusUdgivet - 2019

ID: 59158539