Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Taking gastro-surveillance into the 21st century

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The challenges of defining sample adequacy in an era of HPV based cervical screening

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Aseptic meningitis outbreak associated with echovirus 4 in Northern Europe in 2013-2014

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Clinical validation of the Cobas 4800 HPV assay using cervical samples in SurePath medium under the VALGENT4 framework

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Clinical performance of the HPV-Risk assay on cervical samples in SurePath medium using the VALGENT-4 panel

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Epidemiologi, klinik og behandling af SARS-CoV-2 infektion hos børn

    Publikation: Bidrag til tidsskriftReviewpeer review

  2. First wave of COVID-19 hospital admissions in Denmark: a Nationwide population-based cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Risk of COVID-19 in health-care workers in Denmark: an observational cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. A Systematic Review of Clinical Practice Guidelines for the Diagnosis and Management of Bronchiolitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Enteric viruses, particularly rotaviruses and noroviruses, are leading causes of gastroenteritis worldwide. Human rotaviruses are ubiquitous and globally almost every child has been infected by 3-5 years of age. Noroviruses affect people of all ages and is the leading cause of foodborne outbreaks. Rota- and noroviruses account for ˜40% and ˜17% of diarrhea-associated hospitalizations, and ˜200,000 deaths annually respectively, with most deaths occurring in developing countries. Two rotavirus vaccines have currently been implemented in ˜95 countries and several norovirus vaccine candidates are currently in development and/or clinical testing. Surveillance of enteric viruses is an important part of outbreak investigations as well as pre- and post-vaccine impact studies but is even in developed countries often limited to investigation of sporadic cases or comprehensive outbreaks. Conventional methods for enteric virus detection and subtyping relies on standard RT-PCR methods, supplemented with Sanger-sequencing. However, for viruses with even moderate mutationrates, PCR-based-typing of only limited parts of the virus genome is challenging and requires regular update of primers. Full-genomecharacterization technologies based on sequence independent methods based on next generation sequencing (NGS), have demonstrated great potential for enteric virus detection and/or typing in both clinical and environmental samples. However, cost-benefits must balance for such methods to be widely accepted for public health purposes. In Europe as also globally, routine use of NGS-methods for surveillance of enteric viruses is currently limited to few national public health laboratories. What important lessons can be learned from these and what is the future of NGS-based surveillance?

OriginalsprogEngelsk
TidsskriftJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Vol/bind117
Sider (fra-til)43-48
Antal sider6
ISSN1386-6532
DOI
StatusUdgivet - 2019

ID: 57339823