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Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats

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Andersen, Sofie Bech ; Taghavi, Iman ; Søgaard, Stinne Byrholdt ; Hoyos, Carlos Armando Villagómez ; Nielsen, Michael Bachmann ; Jensen, Jørgen Arendt ; Sørensen, Charlotte Mehlin. / Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats. I: Diagnostics. 2022 ; Bind 12, Nr. 5.

Bibtex

@article{ee0768c101d34fe38b04fabf12f27465,
title = "Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats",
abstract = "Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclusively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.",
keywords = "contrast-enhanced ultrasound, kidneys, microvascular flow, prazosin, Sprague Dawley rats, ultrasound localization microscopy",
author = "Andersen, {Sofie Bech} and Iman Taghavi and S{\o}gaard, {Stinne Byrholdt} and Hoyos, {Carlos Armando Villag{\'o}mez} and Nielsen, {Michael Bachmann} and Jensen, {J{\o}rgen Arendt} and S{\o}rensen, {Charlotte Mehlin}",
year = "2022",
month = apr,
day = "28",
doi = "10.3390/diagnostics12051111",
language = "English",
volume = "12",
journal = "Diagnostics",
issn = "2075-4418",
publisher = "M D P I AG",
number = "5",

}

RIS

TY - JOUR

T1 - Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats

AU - Andersen, Sofie Bech

AU - Taghavi, Iman

AU - Søgaard, Stinne Byrholdt

AU - Hoyos, Carlos Armando Villagómez

AU - Nielsen, Michael Bachmann

AU - Jensen, Jørgen Arendt

AU - Sørensen, Charlotte Mehlin

PY - 2022/4/28

Y1 - 2022/4/28

N2 - Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclusively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.

AB - Super-resolution ultrasound imaging, based on the localization and tracking of single intravascular microbubbles, makes it possible to map vessels below 100 µm. Microbubble velocities can be estimated as a surrogate for blood velocity, but their clinical potential is unclear. We investigated if a decrease in microbubble velocity in the arterial and venous beds of the renal cortex, outer medulla, and inner medulla was detectable after intravenous administration of the α1-adrenoceptor antagonist prazosin. The left kidneys of seven rats were scanned with super-resolution ultrasound for 10 min before, during, and after prazosin administration using a bk5000 ultrasound scanner and hockey-stick probe. The super-resolution images were manually segmented, separating cortex, outer medulla, and inner medulla. Microbubble tracks from arteries/arterioles were separated from vein/venule tracks using the arterial blood flow direction. The mean microbubble velocities from each scan were compared. This showed a significant prazosin-induced velocity decrease only in the cortical arteries/arterioles (from 1.59 ± 0.38 to 1.14 ± 0.31 to 1.18 ± 0.33 mm/s, p = 0.013) and outer medulla descending vasa recta (from 0.70 ± 0.05 to 0.66 ± 0.04 to 0.69 ± 0.06 mm/s, p = 0.026). Conclusively, super-resolution ultrasound imaging makes it possible to detect and differentiate microbubble velocity responses to prazosin simultaneously in the renal cortical and medullary vascular beds.

KW - contrast-enhanced ultrasound

KW - kidneys

KW - microvascular flow

KW - prazosin

KW - Sprague Dawley rats

KW - ultrasound localization microscopy

UR - http://www.scopus.com/inward/record.url?scp=85129668213&partnerID=8YFLogxK

U2 - 10.3390/diagnostics12051111

DO - 10.3390/diagnostics12051111

M3 - Journal article

C2 - 35626267

VL - 12

JO - Diagnostics

JF - Diagnostics

SN - 2075-4418

IS - 5

M1 - 1111

ER -

ID: 78307431