Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Studies of the binding of ficolin-2 and ficolin-3 from the complement lectin pathway to Leptospira biflexa, Pasteurella pneumotropica and Diarrheagenic Escherichia coli

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The host response to bacterial bone infection involves a local upregulation of several acute phase proteins

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Expression of complement C3, C5, C3aR and C5aR1 genes in resting and activated CD4+ T cells

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Reconstitution of Th17, Tc17 and Treg cells after paediatric haematopoietic stem cell transplantation: Impact of interleukin-7

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Alveolar recruitment of ficolin-3 in response to acute pulmonary inflammation in humans

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Prediction of survival in amyotrophic lateral sclerosis: a nationwide, Danish cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Functional Effects of Receptor-Binding Domain Mutations of SARS-CoV-2 B.1.351 and P.1 Variants

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Effect of immunoglobulin G on cytokine response in necrotising soft-tissue infection: a post-hoc analysis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Alfredo Sahagún-Ruiz
  • Leandro Carvalho Dantas Breda
  • Mónica Marcela Castiblanco Valencia
  • Waldir P Elias
  • Lea Munthe-Fog
  • Peter Garred
  • Angela Silva Barbosa
  • Lourdes Isaac
Vis graf over relationer

Ficolins recognize pathogen associated molecular patterns and activate the lectin pathway of complement system. However, our knowledge regarding pathogen recognition of human ficolins is still limited. We therefore set out to explore and investigate the possible interactions of the two main serum ficolins, ficolin-2 and ficolin-3 with different Gram-negative bacteria. We used recombinant ficolin molecules and normal human serum, which were detected with anti-ficolin monoclonal antibodies. In addition we investigated the capacity of these pathogens to activate the lectin pathway of complement system. We show for the first time that human ficolin-2 recognizes the nonpathogenic spirochete Leptospira biflexa serovar Patoc, but not the pathogenic Leptospira interrogans serovar Kennewicki strain Fromm. Additionally, human ficolin-2 and ficolin-3 recognize pathogenic Pasteurella pneumotropica, enteropathogenic Escherichia coli (EPEC) serotype O111ab:H2 and enteroaggregative E. coli (EAEC) serogroup O71 but not four enterohemorrhagic E. coli, three EPEC, three EAEC and two nonpathogenic E. coli strains (DH5α and HB101). The lectin pathway was activated by Pasteurella pneumotropica, EPEC O111ab:H2 and EAEC O71 after incubation with C1q depleted human serum. In conclusion, this study provide novel insight in the binding and complement activating capacity of the lectin pathway initiation molecules ficolin-2 and ficolin-3 towards relevant Gram-negative pathogens of pathophysiological relevance.

OriginalsprogEngelsk
TidsskriftImmunobiology
Vol/bind220
Udgave nummer10
Sider (fra-til)1177-85
Antal sider9
ISSN0171-2985
DOI
StatusUdgivet - okt. 2015

ID: 45692601