Spectral components of cytosolic [Ca2+] spiking in neurons

J Kardos, N Szilágyi, G Juhász, B Belhage, A Schousboe

5 Citationer (Scopus)

Abstract

We show here, by means of evolutionary spectral analysis and synthesis of cytosolic Ca2+ ([Ca2+]c) spiking observed at the single cell level using digital imaging fluorescence microscopy of fura-2-loaded mouse cerebellar granule cells in culture, that [Ca2+]c spiking can be resolved into evolutionary spectra of a characteristic set of frequencies. Non-delayed small spikes on top of sustained [Ca2+]c were synthesized by a main component frequency, 0.132+/-0.012 Hz, showing its maximal amplitude in phase with the start of depolarization (25 mM KCI) combined with caffeine (10 mM) application. Delayed complex responses of large [Ca2+]c spiking observed in cells from a different set of cultures were synthesized by a set of frequencies within the range 0.018-0.117 Hz. Differential frequency patterns are suggested as characteristics of the [Ca2+]c spiking responses of neurons under different conditions.
OriginalsprogEngelsk
TidsskriftNeuroReport
Vol/bind9
Udgave nummer4
Sider (fra-til)721-4
Antal sider4
ISSN0959-4965
StatusUdgivet - 9 mar. 1998

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