Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

Beatrice Dyring-Andersen, Marianne Bengtson Løvendorf, Fabian Coscia, Alberto Santos, Line Bruun Pilgaard Møller, Ana R Colaço, Lili Niu, Michael Bzorek, Sophia Doll, Jørgen Lock Andersen, Rachael A Clark, Lone Skov, Marcel B M Teunissen, Matthias Mann

Abstract

Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research ( https://skin.science/ ).

OriginalsprogEngelsk
Artikelnummer5587
TidsskriftNature Communications
Vol/bind11
Udgave nummer1
Sider (fra-til)5587
ISSN2041-1722
DOI
StatusUdgivet - 5 nov. 2020

Fingeraftryk

Dyk ned i forskningsemnerne om 'Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin'. Sammen danner de et unikt fingeraftryk.

Citationsformater