TY - JOUR
T1 - Somatostatin attenuates the hyperthermia induced increase in neutrophil concentration
AU - Kappel, M.
AU - Poulsen, T. D.
AU - Hansen, M. B.
AU - Galbo, H.
AU - Pedersen, B. K.
N1 - Funding Information:
Acknowledgements The excellent technical assistance of Lars Bra-agaard, Hanne Villumsen and Ruth Rousing is acknowledged. The work was supported by the Danish Rheumatism Association, the Danish Foundation for the Advancement of Medical Science, the Novo’s Foundations Committee, Kong Christian den Tiendes Fond and the Danish National Research Foundation no. 501–14. The Department of Clinical Biochemistry, Rigshospitalet, is acknowledged for the measurements of the leucocyte counts. Finally, we are grateful to Dr. Peter Norsk, The Danish Aerospace Medical Centre of Research (DAMEC research), Rigshospitalet, for putting the water immersion tank at our disposal.
PY - 1997/12
Y1 - 1997/12
N2 - This study was designed to test the hypothesis that the immune changes seen during in vivo whole body hyperthermia are mediated by elevations in the plasma concentrations of either catecholamines, growth hormone or β-endorphins. Eight healthy volunteers were immersed in a hot water bath (WI; water temperature 39.5°C) for 2 h during which their rectal temperature rose to 39.5°C. In a single blind, randomized, cross-over study the stress hormone effects were blocked one at a time by administration of propranolol, somatostatin or naloxone, the results were compared to those obtained during saline infusion (control). Blood samples were collected before, at the end of 2 h of WI (body temperature 39.5°C), and 2 h later. Hormone blockade did not abolish the hyperthermia-induced recruitment of natural killer (NK) cells to the blood, and no influence was observed on the percentages or concentrations of any other subpopulations of blood mononuclear cells, except that the number of cluster designation (CD)3+ cells slightly increased after hyperthermia only in the propranolol experiment. Furthermore, the NK cell activity, both unstimulated and interferon-α or interleukin-2 stimulated, did nor differ from the control situation. It is of interest, however, that somatostatin partly abolished the hyperthermia induced increase in the neutrophil number. Based on these data and previous results showing that growth hormone infusion increases the concentration of neutrophils in the blood, it is suggested that growth hormone is at least partly responsible for hyperthermia induced neutrocytosis.
AB - This study was designed to test the hypothesis that the immune changes seen during in vivo whole body hyperthermia are mediated by elevations in the plasma concentrations of either catecholamines, growth hormone or β-endorphins. Eight healthy volunteers were immersed in a hot water bath (WI; water temperature 39.5°C) for 2 h during which their rectal temperature rose to 39.5°C. In a single blind, randomized, cross-over study the stress hormone effects were blocked one at a time by administration of propranolol, somatostatin or naloxone, the results were compared to those obtained during saline infusion (control). Blood samples were collected before, at the end of 2 h of WI (body temperature 39.5°C), and 2 h later. Hormone blockade did not abolish the hyperthermia-induced recruitment of natural killer (NK) cells to the blood, and no influence was observed on the percentages or concentrations of any other subpopulations of blood mononuclear cells, except that the number of cluster designation (CD)3+ cells slightly increased after hyperthermia only in the propranolol experiment. Furthermore, the NK cell activity, both unstimulated and interferon-α or interleukin-2 stimulated, did nor differ from the control situation. It is of interest, however, that somatostatin partly abolished the hyperthermia induced increase in the neutrophil number. Based on these data and previous results showing that growth hormone infusion increases the concentration of neutrophils in the blood, it is suggested that growth hormone is at least partly responsible for hyperthermia induced neutrocytosis.
KW - Growth hormone
KW - Hormones
KW - Human
KW - Hyperthermia
KW - Immunology
UR - http://www.scopus.com/inward/record.url?scp=0031888113&partnerID=8YFLogxK
U2 - 10.1007/s004210050313
DO - 10.1007/s004210050313
M3 - Journal article
C2 - 9459535
AN - SCOPUS:0031888113
SN - 0301-5548
VL - 77
SP - 149
EP - 156
JO - European Journal of Applied Physiology and Occupational Physiology
JF - European Journal of Applied Physiology and Occupational Physiology
IS - 1-2
ER -