@article{2dd23d41f5d24c3da8c3fa6f7c41c493,
title = "Skeletal muscle structural lipids improve during weight-maintenance after a very low calorie dietary intervention",
abstract = "BACKGROUND: The objective was to investigate in a group of obese subjects the course in skeletal muscle phospholipid (SMPL) fatty acids (FA) during a 24-weeks weight maintenance program, which was preceded by a successful very low calorie dietary intervention (VLCD). Special focus was addressed to SMPL omega-3 FA, which is a lipid entity that influences insulin action. METHODS: Nine obese subjects (BMI = 35.7 +/- 1.0 kg/m(2)), who had completed an 8 weeks VLCD (weight-loss = -9.7 +/- 1.6 kg, P < 0.001), had obtained skeletal muscle biopsies (vastus lateralis) before and after a dietician-guided 24-weeks weight-maintenance program (-1.2 +/- 1.5 kg, P = ns). SMPL FA composition was determined by gas liquid chromatography. During the preceding VLCD, insulin sensitivity (HOMA-IR) and glycemic control (HbA1c) improved but no change in SMPL omega-3 FA was observed. During the weight-maintenance program five subjects received the pancreas lipase inhibitor Orlistat 120 mg t.i.d. versus placebo. RESULTS: HOMA-IR and HbA1c stabilized and SMPL total omega-3 FA, docosahexaenoic acid and ratio of n-3/n-6 polyunsaturated FA increased by 24% (P < 0.01), 35% (P < 0.02) and 26% (P < 0.01), respectively, whereas saturated and monounsaturated FA did not change. Plasma total-cholesterol and LDL-cholesterol, which decreased during the VLCD, reverted to pre-VLCD levels (P < 0.01). Orlistat therapy was associated with weight-loss (P < 0.05), trends for better glycemic control (P = 0.15) and greater increase in SMPL docosahexaenoic acid (P = 0.12) but similar reversal of plasma cholesterols compared to placebo. CONCLUSION: The data are consistent with the notion that greater SMPL omega-3 FA obtained during a weight-maintenance program may play a role for preserving insulin sensitivity and glycemic control being generated during a preceding VLCD.",
keywords = "Body Mass Index, Caloric Restriction, Diet, Reducing, Docosahexaenoic Acids, Double-Blind Method, Eicosapentaenoic Acid, Enzyme Inhibitors, Fatty Acids, Female, Follow-Up Studies, Humans, Insulin Resistance, Lactones, Lipase, Lipids, Middle Aged, Muscle, Skeletal, Obesity, Phospholipids, Pilot Projects, Triglycerides",
author = "Haugaard, {Steen B} and Allan Vaag and Huiling Mu and Sten Madsbad",
year = "2009",
doi = "10.1186/1476-511X-8-34",
language = "English",
volume = "8",
pages = "34",
journal = "Lipids in Health and Disease",
publisher = "BioMed Central Ltd",
}