Abstract
BACKGROUND AIMS: The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).
METHODS: To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.
RESULTS: This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.
CONCLUSIONS: Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.
Originalsprog | Engelsk |
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Tidsskrift | Cytotherapy |
Vol/bind | 16 |
Udgave nummer | 8 |
Sider (fra-til) | 1117-20 |
Antal sider | 4 |
ISSN | 1465-3249 |
DOI | |
Status | Udgivet - aug. 2014 |