TY - JOUR
T1 - Short polystyrene monolith-fritted micro-liquid chromatography columns for rapid isocratic analysis of pharmaceuticals direct from plasma
AU - Legido-Quigley, Cristina
AU - Smith, Norman W
PY - 2006/6
Y1 - 2006/6
N2 - The manufacture of micro-HPLC columns with combined stationary phases, a body of 3.5-microm XTerra-C18 particles, and poly(styrene-divinylbenzene) (PS-DVB) frits is described in detail. The efficiency of the columns was assessed by rapid separation of neutral and acid compound mixtures. Direct analysis of some pharmaceuticals in plasma resulted in lower limits of detection (LOD) for salmeterol xinofate of 12.5 nanograms on-column.
AB - The manufacture of micro-HPLC columns with combined stationary phases, a body of 3.5-microm XTerra-C18 particles, and poly(styrene-divinylbenzene) (PS-DVB) frits is described in detail. The efficiency of the columns was assessed by rapid separation of neutral and acid compound mixtures. Direct analysis of some pharmaceuticals in plasma resulted in lower limits of detection (LOD) for salmeterol xinofate of 12.5 nanograms on-column.
KW - Albuterol/analogs & derivatives
KW - Androstadienes/blood
KW - Chromatography, High Pressure Liquid/instrumentation
KW - Fluticasone
KW - Polystyrenes/chemistry
KW - Salmeterol Xinafoate
KW - Time Factors
U2 - 10.1007/s00216-006-0457-7
DO - 10.1007/s00216-006-0457-7
M3 - Journal article
C2 - 16741767
SN - 1618-2642
VL - 385
SP - 686
EP - 691
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 4
ER -