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Serum levels of INSL3, AMH, Inhibin B and Testosterone during pubertal transition in healthy boys

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@article{d3ff83b3c2954611bd25ce44a708e52c,
title = "Serum levels of INSL3, AMH, Inhibin B and Testosterone during pubertal transition in healthy boys",
abstract = "IntroductionInsulin-like factor 3 (INSL3) is a promising marker of Leydig cell function with potentially high clinical relevance. Limited data of INSL3 levels in relation to other reproductive hormones in healthy pubertal boys exist.AimTo evaluate longitudinal serum changes in INSL3 compared to luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, inhibin B, and anti-M{\"u}llerian hormone (AMH) during puberty in healthy boys.MethodsTen boys were included from the longitudinal part of the COPENHAGEN Puberty Study. Pubertal evaluation, including testicular volume, was performed and blood samples drawn every 6 months for 5 years. Serum concentrations of testosterone were determined by a newly developed LC-MS/MS method, and serum concentrations of INSL3, AMH, inhibin B, FSH and LH, respectively, were determined by validated immunoassays.ResultsSerum INSL3 levels increased progressively with increasing age, pubertal onset and testicular volume. In six of ten boys, LH increased prior to the first observed increase in INSL3. In the remaining four boys, the increase in LH and INSL3 was observed at the same examination. The increases in serum concentrations of LH, testosterone and INSL3 were not parallel or in ordered succession and varied inter-individuallyConclusionWe demonstrated that INSL3 concentrations were tightly associated with pubertal onset and increasing testicular volume. However, the pubertal increases in LH, INSL3, and testosterone concentrations were not entirely parallel, suggesting that INSL3 and testosterone may be regulated differently. Thus, we speculate that INSL3 provides additional information on Leydig cell differentiation and function during puberty compared with traditional markers of testicular function.",
author = "{Lindhardt Johansen}, Marie and Ravinder Anand-Ivell and Annette Mouritsen and Hagen, {Casper P} and Mieritz, {Mikkel Grunnet} and Tue S{\o}eborg and Johannsen, {Trine Holm} and Main, {Katharina M} and Anna-Maria Andersson and Richard Ivell and Anders Juul",
year = "2014",
month = "1",
day = "23",
doi = "10.1530/REP-13-0435",
language = "English",
volume = "147",
pages = "529--535",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd",

}

RIS

TY - JOUR

T1 - Serum levels of INSL3, AMH, Inhibin B and Testosterone during pubertal transition in healthy boys

AU - Lindhardt Johansen, Marie

AU - Anand-Ivell, Ravinder

AU - Mouritsen, Annette

AU - Hagen, Casper P

AU - Mieritz, Mikkel Grunnet

AU - Søeborg, Tue

AU - Johannsen, Trine Holm

AU - Main, Katharina M

AU - Andersson, Anna-Maria

AU - Ivell, Richard

AU - Juul, Anders

PY - 2014/1/23

Y1 - 2014/1/23

N2 - IntroductionInsulin-like factor 3 (INSL3) is a promising marker of Leydig cell function with potentially high clinical relevance. Limited data of INSL3 levels in relation to other reproductive hormones in healthy pubertal boys exist.AimTo evaluate longitudinal serum changes in INSL3 compared to luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, inhibin B, and anti-Müllerian hormone (AMH) during puberty in healthy boys.MethodsTen boys were included from the longitudinal part of the COPENHAGEN Puberty Study. Pubertal evaluation, including testicular volume, was performed and blood samples drawn every 6 months for 5 years. Serum concentrations of testosterone were determined by a newly developed LC-MS/MS method, and serum concentrations of INSL3, AMH, inhibin B, FSH and LH, respectively, were determined by validated immunoassays.ResultsSerum INSL3 levels increased progressively with increasing age, pubertal onset and testicular volume. In six of ten boys, LH increased prior to the first observed increase in INSL3. In the remaining four boys, the increase in LH and INSL3 was observed at the same examination. The increases in serum concentrations of LH, testosterone and INSL3 were not parallel or in ordered succession and varied inter-individuallyConclusionWe demonstrated that INSL3 concentrations were tightly associated with pubertal onset and increasing testicular volume. However, the pubertal increases in LH, INSL3, and testosterone concentrations were not entirely parallel, suggesting that INSL3 and testosterone may be regulated differently. Thus, we speculate that INSL3 provides additional information on Leydig cell differentiation and function during puberty compared with traditional markers of testicular function.

AB - IntroductionInsulin-like factor 3 (INSL3) is a promising marker of Leydig cell function with potentially high clinical relevance. Limited data of INSL3 levels in relation to other reproductive hormones in healthy pubertal boys exist.AimTo evaluate longitudinal serum changes in INSL3 compared to luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, inhibin B, and anti-Müllerian hormone (AMH) during puberty in healthy boys.MethodsTen boys were included from the longitudinal part of the COPENHAGEN Puberty Study. Pubertal evaluation, including testicular volume, was performed and blood samples drawn every 6 months for 5 years. Serum concentrations of testosterone were determined by a newly developed LC-MS/MS method, and serum concentrations of INSL3, AMH, inhibin B, FSH and LH, respectively, were determined by validated immunoassays.ResultsSerum INSL3 levels increased progressively with increasing age, pubertal onset and testicular volume. In six of ten boys, LH increased prior to the first observed increase in INSL3. In the remaining four boys, the increase in LH and INSL3 was observed at the same examination. The increases in serum concentrations of LH, testosterone and INSL3 were not parallel or in ordered succession and varied inter-individuallyConclusionWe demonstrated that INSL3 concentrations were tightly associated with pubertal onset and increasing testicular volume. However, the pubertal increases in LH, INSL3, and testosterone concentrations were not entirely parallel, suggesting that INSL3 and testosterone may be regulated differently. Thus, we speculate that INSL3 provides additional information on Leydig cell differentiation and function during puberty compared with traditional markers of testicular function.

U2 - 10.1530/REP-13-0435

DO - 10.1530/REP-13-0435

M3 - Journal article

VL - 147

SP - 529

EP - 535

JO - Reproduction

JF - Reproduction

SN - 1470-1626

ER -

ID: 42608324