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Serum Anticytokine Autoantibody Levels Are Not Increased in Hidradenitis Suppurativa: A Case-Control Pilot Study

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@article{cb6fbcf77b184358aa8c49c03676abfd,
title = "Serum Anticytokine Autoantibody Levels Are Not Increased in Hidradenitis Suppurativa: A Case-Control Pilot Study",
abstract = "BACKGROUND: Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α.METHOD: Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab')2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples.RESULTS: No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied.DISCUSSION: The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.",
keywords = "Journal Article",
author = "{Theut Riis}, Peter and {von Stemann}, {Jakob Hjorth} and {Kj{\ae}rsgaard Andersen}, Rune and Hansen, {Morten Bagge} and Jemec, {Gregor B E}",
note = "{\circledC} 2017 S. Karger AG, Basel.",
year = "2017",
doi = "10.1159/000475925",
language = "English",
volume = "233",
pages = "126--128",
journal = "Dermatology",
issn = "1018-8665",
publisher = "S./Karger AG",
number = "2-3",

}

RIS

TY - JOUR

T1 - Serum Anticytokine Autoantibody Levels Are Not Increased in Hidradenitis Suppurativa

T2 - A Case-Control Pilot Study

AU - Theut Riis, Peter

AU - von Stemann, Jakob Hjorth

AU - Kjærsgaard Andersen, Rune

AU - Hansen, Morten Bagge

AU - Jemec, Gregor B E

N1 - © 2017 S. Karger AG, Basel.

PY - 2017

Y1 - 2017

N2 - BACKGROUND: Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α.METHOD: Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab')2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples.RESULTS: No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied.DISCUSSION: The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.

AB - BACKGROUND: Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α.METHOD: Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab')2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples.RESULTS: No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied.DISCUSSION: The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.

KW - Journal Article

U2 - 10.1159/000475925

DO - 10.1159/000475925

M3 - Journal article

VL - 233

SP - 126

EP - 128

JO - Dermatology

JF - Dermatology

SN - 1018-8665

IS - 2-3

ER -

ID: 52335463