TY - JOUR
T1 - Sequence analysis of the Legionella micdadei groELS operon
AU - Hindersson, P
AU - Høiby, N
AU - Bangsborg, J
PY - 1991/1/1
Y1 - 1991/1/1
N2 - A 2.7 kb DNA fragment encoding the 60 kDa common antigen (CA) and a 13 kDa protein of Legionella micdadei was sequenced. Two open reading frames of 57,677 and 10,456 Da were identified, corresponding to the heat shock proteins GroEL and GroES, respectively. Typical -35, -10, and Shine-Dalgarno heat shock expression signals were identified upstream of the L. micdadei groEL gene. Further upstream, a poly-T region, also a feature of the sigma 32-regulated Escherichia coli groELS heat shock operon, was found. Despite the high degree of homology of the expression signals in E. coli and L. micdadei, Western blot analysis with an L. micdadei specific anti-groEL antibody did not reveal a significant increase in the amount of the GroEL protein during heat shock in L. micdadei or in the recombinant E. coli expressing L. micdadei GroEL.
AB - A 2.7 kb DNA fragment encoding the 60 kDa common antigen (CA) and a 13 kDa protein of Legionella micdadei was sequenced. Two open reading frames of 57,677 and 10,456 Da were identified, corresponding to the heat shock proteins GroEL and GroES, respectively. Typical -35, -10, and Shine-Dalgarno heat shock expression signals were identified upstream of the L. micdadei groEL gene. Further upstream, a poly-T region, also a feature of the sigma 32-regulated Escherichia coli groELS heat shock operon, was found. Despite the high degree of homology of the expression signals in E. coli and L. micdadei, Western blot analysis with an L. micdadei specific anti-groEL antibody did not reveal a significant increase in the amount of the GroEL protein during heat shock in L. micdadei or in the recombinant E. coli expressing L. micdadei GroEL.
KW - Amino Acid Sequence
KW - Bacterial Proteins/genetics
KW - Base Sequence
KW - Chaperonin 60
KW - DNA, Bacterial/chemistry
KW - Escherichia coli/genetics
KW - Gene Expression Regulation, Bacterial
KW - Heat-Shock Proteins/genetics
KW - Legionella/genetics
KW - Molecular Sequence Data
KW - Molecular Weight
KW - Open Reading Frames
KW - Operon
KW - Regulatory Sequences, Nucleic Acid
KW - Restriction Mapping
KW - Sequence Homology, Nucleic Acid
U2 - 10.1016/0378-1097(91)90009-y
DO - 10.1016/0378-1097(91)90009-y
M3 - Journal article
C2 - 1672279
SN - 0378-1097
VL - 61
SP - 31
EP - 38
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -