Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing

Karen L Nielsen; Paul A Godfrey; Marc Stegger; Paal S Andersen; Michael Feldgarden; Niels Frimodt-Møller

doi:10.1016/j.mimet.2014.05.018

Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing

Karen L Nielsen, Paul A Godfrey, Marc Stegger, Paal S Andersen, Michael Feldgarden, Niels Frimodt-Møller

Klinisk Mikrobiologisk Afdeling AHH

29 Citationer (Scopus)

Abstract

Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

Originalsprog	Engelsk
Tidsskrift	Journal of Microbiological Methods
Vol/bind	103
Sider (fra-til)	101-3
Antal sider	3
ISSN	0167-7012
DOI	https://doi.org/10.1016/j.mimet.2014.05.018
Status	Udgivet - aug. 2014

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abstract = "Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.",

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T1 - Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD)

T2 - Evaluation by whole genome sequencing

AU - Nielsen, Karen L

AU - Godfrey, Paul A

AU - Stegger, Marc

AU - Andersen, Paal S

AU - Feldgarden, Michael

AU - Frimodt-Møller, Niels

PY - 2014/8

Y1 - 2014/8

N2 - Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

AB - Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

UR - https://www.scopus.com/pages/publications/84904692085

U2 - 10.1016/j.mimet.2014.05.018

DO - 10.1016/j.mimet.2014.05.018

M3 - Journal article

C2 - 24912108

SN - 0167-7012

VL - 103

SP - 101

EP - 103

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing

Abstract

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