TY - JOUR
T1 - Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman
AU - Bøllehuus Hansen, Lasse
AU - Rehfeld, Anders
AU - de Neergaard, Rosanna
AU - Nielsen, John Erik
AU - Iversen, Lea Hedegaard
AU - Boisen, Ida Marie
AU - Mortensen, Li Juel
AU - Lanske, Beate
AU - Almstrup, Kristian
AU - Carlsen, Elisabeth
AU - Berg, Anders Hayden
AU - Jørgensen, Niels
AU - Andersen, Anders Nyboe
AU - Juul, Anders
AU - Blomberg Jensen, Martin
PY - 2017
Y1 - 2017
N2 - CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.SETTING: Tertiary centre for fertility.PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.
AB - CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.SETTING: Tertiary centre for fertility.PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.
U2 - 10.1210/jc.2016-3008
DO - 10.1210/jc.2016-3008
M3 - Journal article
C2 - 27977320
SN - 0021-972X
VL - 102
SP - 950
EP - 961
JO - The Journal of clinical endocrinology and metabolism
JF - The Journal of clinical endocrinology and metabolism
IS - 3
ER -