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Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

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Mauch, Renan Marrichi ; Rossi, Claudio Lucio ; Nolasco da Silva, Marcos Tadeu ; Bianchi Aiello, Talita ; Ribeiro, José Dirceu ; Ribeiro, Antônio Fernando ; Høiby, Niels ; Levy, Carlos Emilio. / Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients. I: Medical Microbiology and Immunology. 2019 ; Bind 208, Nr. 2. s. 205-213.

Bibtex

@article{ec08dee80bbc480185d4648e6d644a2f,
title = "Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients",
abstract = "Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test's ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.",
keywords = "Adolescent, Antibodies, Bacterial/immunology, Child, Child, Preschool, Cystic Fibrosis/complications, Enzyme-Linked Immunosorbent Assay/methods, Female, Follow-Up Studies, Humans, Immunoglobulin A, Secretory/immunology, Infant, Male, Pseudomonas Infections/immunology, Pseudomonas aeruginosa/immunology, Respiratory Tract Infections/immunology, Saliva/immunology, Time Factors",
author = "Mauch, {Renan Marrichi} and Rossi, {Claudio Lucio} and {Nolasco da Silva}, {Marcos Tadeu} and {Bianchi Aiello}, Talita and Ribeiro, {Jos{\'e} Dirceu} and Ribeiro, {Ant{\^o}nio Fernando} and Niels H{\o}iby and Levy, {Carlos Emilio}",
year = "2019",
month = apr,
doi = "10.1007/s00430-019-00578-w",
language = "English",
volume = "208",
pages = "205--213",
journal = "Medical Microbiology and Immunology",
issn = "0300-8584",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients

AU - Mauch, Renan Marrichi

AU - Rossi, Claudio Lucio

AU - Nolasco da Silva, Marcos Tadeu

AU - Bianchi Aiello, Talita

AU - Ribeiro, José Dirceu

AU - Ribeiro, Antônio Fernando

AU - Høiby, Niels

AU - Levy, Carlos Emilio

PY - 2019/4

Y1 - 2019/4

N2 - Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test's ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.

AB - Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Patients who remained Pa-positive had significantly increased sIgA levels than patients who remained Pa-negative, both at the baseline (39.6 U/mL vs. 19.2 U/mL; p = 0.02) and at the end of the follow-up (119.4 U/mL vs. 25.2 U/mL; p < 0.001). No association was found between sIgA levels in saliva and emergence or recurrence of Pa in the LAW. A positive median sIgA result in the first year of follow-up implied up to 12.5-fold increased risk of subsequent Pa exposure in the LAW. Our test detected early changes in the P. aeruginosa LAW status and risk of exposure to P. aeruginosa in the LAW with two years in advance. Comparison with sinus culture is needed to assess the test's ability to identify CF patients in need of a sinus approach for Pa investigation, which could provide opportunities of Pa eradication before its aspiration to the lungs.

KW - Adolescent

KW - Antibodies, Bacterial/immunology

KW - Child

KW - Child, Preschool

KW - Cystic Fibrosis/complications

KW - Enzyme-Linked Immunosorbent Assay/methods

KW - Female

KW - Follow-Up Studies

KW - Humans

KW - Immunoglobulin A, Secretory/immunology

KW - Infant

KW - Male

KW - Pseudomonas Infections/immunology

KW - Pseudomonas aeruginosa/immunology

KW - Respiratory Tract Infections/immunology

KW - Saliva/immunology

KW - Time Factors

U2 - 10.1007/s00430-019-00578-w

DO - 10.1007/s00430-019-00578-w

M3 - Journal article

C2 - 30706137

VL - 208

SP - 205

EP - 213

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

SN - 0300-8584

IS - 2

ER -

ID: 59045700