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Role of Pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion

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@article{f6c604c742734b7faf6aee517c98665f,
title = "Role of Pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion",
abstract = "OBJECTIVES: The purinergic system has not been investigated in detail following ischemia/reperfusion (I/R) injury in the heart. In the present study we focus on both release and response to extracellular adenosine triphosphate (ATP). Pannexin (Panx) channels have been shown to be involved in ATP release from myocytes and can activate P2X1 and P2Y2 receptors on the coronary artery.DESIGN: We applied a well characterized I/R model in rats, with 24 hours of reperfusion. Panx expression in the myocardial tissue was measured with quantitative polymerase chain reaction (qPCR) and flow cytometry. ATP release was detected in situ using luminescence and the vascular response to nucleotides determined in a wire myograph.RESULTS: Here we show that Panx expression is increased after experimental myocardial I/R, leading to an increase in extracellular ATP release, which could be inhibited by probenecid. Functional studies revealed that the P2Y2 receptor dependent contraction is reduced in the coronary artery after I/R, which might be a response to the increased ATP levels.CONCLUSION: We therefore, conclude that the regulation of the arterial purinergic system minimizes coronary contractions following ischemia.",
keywords = "Ischemia/reperfusion, coronary artery, myograph, pannexin, purinergic receptors, Paracrine Communication, Signal Transduction, Vasoconstriction, Adenosine Triphosphate/metabolism, Rats, Sprague-Dawley, Myocardial Infarction/genetics, Animals, Connexins/genetics, Myocardium/metabolism, Nerve Tissue Proteins/genetics, Receptors, Purinergic P2Y2/metabolism, Myocardial Reperfusion Injury/genetics, Coronary Vessels/metabolism, Disease Models, Animal",
author = "Kristiansen, {Sarah Br{\o}gger} and Skovsted, {Gry Freja} and Berchtold, {Lukas Adrian} and Aneta Radziwon-Balicka and Karin Dreisig and Lars Edvinsson and Majid Sheykhzade and Haanes, {Kristian Agmund}",
year = "2018",
doi = "10.1080/14017431.2018.1552793",
language = "English",
volume = "52",
pages = "340--343",
journal = "Scandinavian Cardiovascular Journal",
issn = "1401-7431",
publisher = "Informa Healthcare",
number = "6",

}

RIS

TY - JOUR

T1 - Role of Pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion

AU - Kristiansen, Sarah Brøgger

AU - Skovsted, Gry Freja

AU - Berchtold, Lukas Adrian

AU - Radziwon-Balicka, Aneta

AU - Dreisig, Karin

AU - Edvinsson, Lars

AU - Sheykhzade, Majid

AU - Haanes, Kristian Agmund

PY - 2018

Y1 - 2018

N2 - OBJECTIVES: The purinergic system has not been investigated in detail following ischemia/reperfusion (I/R) injury in the heart. In the present study we focus on both release and response to extracellular adenosine triphosphate (ATP). Pannexin (Panx) channels have been shown to be involved in ATP release from myocytes and can activate P2X1 and P2Y2 receptors on the coronary artery.DESIGN: We applied a well characterized I/R model in rats, with 24 hours of reperfusion. Panx expression in the myocardial tissue was measured with quantitative polymerase chain reaction (qPCR) and flow cytometry. ATP release was detected in situ using luminescence and the vascular response to nucleotides determined in a wire myograph.RESULTS: Here we show that Panx expression is increased after experimental myocardial I/R, leading to an increase in extracellular ATP release, which could be inhibited by probenecid. Functional studies revealed that the P2Y2 receptor dependent contraction is reduced in the coronary artery after I/R, which might be a response to the increased ATP levels.CONCLUSION: We therefore, conclude that the regulation of the arterial purinergic system minimizes coronary contractions following ischemia.

AB - OBJECTIVES: The purinergic system has not been investigated in detail following ischemia/reperfusion (I/R) injury in the heart. In the present study we focus on both release and response to extracellular adenosine triphosphate (ATP). Pannexin (Panx) channels have been shown to be involved in ATP release from myocytes and can activate P2X1 and P2Y2 receptors on the coronary artery.DESIGN: We applied a well characterized I/R model in rats, with 24 hours of reperfusion. Panx expression in the myocardial tissue was measured with quantitative polymerase chain reaction (qPCR) and flow cytometry. ATP release was detected in situ using luminescence and the vascular response to nucleotides determined in a wire myograph.RESULTS: Here we show that Panx expression is increased after experimental myocardial I/R, leading to an increase in extracellular ATP release, which could be inhibited by probenecid. Functional studies revealed that the P2Y2 receptor dependent contraction is reduced in the coronary artery after I/R, which might be a response to the increased ATP levels.CONCLUSION: We therefore, conclude that the regulation of the arterial purinergic system minimizes coronary contractions following ischemia.

KW - Ischemia/reperfusion

KW - coronary artery

KW - myograph

KW - pannexin

KW - purinergic receptors

KW - Paracrine Communication

KW - Signal Transduction

KW - Vasoconstriction

KW - Adenosine Triphosphate/metabolism

KW - Rats, Sprague-Dawley

KW - Myocardial Infarction/genetics

KW - Animals

KW - Connexins/genetics

KW - Myocardium/metabolism

KW - Nerve Tissue Proteins/genetics

KW - Receptors, Purinergic P2Y2/metabolism

KW - Myocardial Reperfusion Injury/genetics

KW - Coronary Vessels/metabolism

KW - Disease Models, Animal

UR - http://www.scopus.com/inward/record.url?scp=85059622878&partnerID=8YFLogxK

U2 - 10.1080/14017431.2018.1552793

DO - 10.1080/14017431.2018.1552793

M3 - Journal article

C2 - 30481075

VL - 52

SP - 340

EP - 343

JO - Scandinavian Cardiovascular Journal

JF - Scandinavian Cardiovascular Journal

SN - 1401-7431

IS - 6

ER -

ID: 55802007