Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital

Review of injection techniques for spermatogonial stem cell transplantation

Publikation: Bidrag til tidsskriftReviewForskningpeer review


  1. A systematic review and meta-analysis on the association between ICSI and chromosome abnormalities

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. False hope for patients with Klinefelter syndrome on cryopreservation of spermatogonia at younger ages

    Publikation: Bidrag til tidsskriftLetterForskningpeer review

  3. Immediate versus postponed frozen embryo transfer after IVF/ICSI: a systematic review and meta-analysis

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  4. The health of children conceived by ART: 'the chicken or the egg?'

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. How parents express their worry in calls to a medical helpline: a mixed methods study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. "We can't do without it": Parent and call-handler experiences of video triage of children at a medical helpline

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Intestinal perfusion assessed by quantitative fluorescence angiography in piglets with necrotizing enterocolitis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND: Although the prognosis of childhood cancer survivors has increased dramatically during recent years, chemotherapy and radiation treatments for cancer and other conditions may lead to permanent infertility in prepubertal boys. Recent developments have shown that spermatogonial stem cell (SSC) transplantation may be a hope for restoring fertility in adult survivors of childhood cancers. For this reason, several centres around the world are collecting and cryopreserving testicular tissue or cells anticipating that, in the near future, some patients will return for SSC transplantation. This review summarizes the current knowledge and utility of SSC transplantation techniques.

OBJECTIVE AND RATIONALE: The aim of this narrative review is to provide an overview of the currently used experimental injection techniques for SSC transplantation in animal and human testes. This is crucial in understanding and determining the role of the different techniques necessary for successful transplantation.

SEARCH METHODS: A comprehensive review of peer-reviewed publications on this topic was performed using the PubMed and Google Scholar databases. The search was limited to English language work and studies between 1994 (from the first study on SSC transplantation) and April 2019. Key search terms included mouse, rat, boar, ram, dog, sheep, goat, cattle, monkey, human, cadaver, testes, SSC transplantation, injection and technique.

OUTCOMES: This review provides an extensive clinical overview of the current research in the field of human SSC transplantation. Rete testis injection with ultrasonography guidance currently seems the most promising injection technique thus far; however, the ability to draw clear conclusions is limited due to long ischemia time of cadaver testis, the relatively decreased volume of the testis, the diminishing size of seminiferous tubules, a lack of intratesticular pressure and leakage into the interstitium during the injection on human cadaver testis. Current evidence does not support improved outcomes from multiple infusions through the rete testes. Overall, further optimization is required to increase the efficiency and safety of the infusion method.

WIDER IMPLICATIONS: Identifying a favourable injection method for SSC transplantation will provide insight into the mechanisms of successful assisted human reproduction. Future research could focus on reducing leakage and establishing the optimal infusion cell concentrations and pressure.

TidsskriftHuman Reproduction Update
Udgave nummer3
Sider (fra-til)368-391
Antal sider24
StatusUdgivet - 15 apr. 2020

Bibliografisk note

© The Author(s) 2020. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail:

ID: 59545713