Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech; Reno Debets; Per Thor Straten; Marlies J W Peeters

doi:10.1007/978-1-0716-3593-3_3

Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech, Reno Debets, Per Thor Straten, Marlies J W Peeters^*

^*Corresponding author af dette arbejde

Center for Cancer Immune Therapy (CCIT), Department of Oncology, Copenhagen University Hospital , Herlev, Denmark.

Abstract

Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

Originalsprog	Engelsk
Tidsskrift	Methods in Molecular Biology
Vol/bind	2748
Sider (fra-til)	29-39
Antal sider	11
ISSN	1064-3745
DOI	https://doi.org/10.1007/978-1-0716-3593-3_3
Status	Udgivet - 2024

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abstract = "Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.",

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T1 - Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

AU - Rahbech, Anne

AU - Debets, Reno

AU - Thor Straten, Per

AU - Peeters, Marlies J W

PY - 2024

Y1 - 2024

N2 - Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

AB - Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

KW - Cytolysis

KW - Retrovirus

KW - T cells

KW - Transduction

KW - xCELLigence

UR - https://www.scopus.com/pages/publications/85179643842

U2 - 10.1007/978-1-0716-3593-3_3

DO - 10.1007/978-1-0716-3593-3_3

M3 - Journal article

C2 - 38070105

SN - 1064-3745

VL - 2748

SP - 29

EP - 39

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Abstract

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