Retroviral Transduction of Human Primary T Cells Followed by Real-Time T-Cell-Mediated Cancer Cell Cytolysis Analysis

Anne Rahbech, Reno Debets, Per Thor Straten, Marlies J W Peeters*

*Corresponding author af dette arbejde

Abstract

Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell-cancer cell interactions, such as cytolysis analysis using xCELLigence technology.

OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind2748
Sider (fra-til)29-39
Antal sider11
ISSN1064-3745
DOI
StatusUdgivet - 2024

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