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Region Hovedstaden - en del af Københavns Universitetshospital
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Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Clinical impact of leukemic blast heterogeneity at diagnosis in cytogenetic intermediate-risk acute myeloid leukemia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Multiparametric flow cytometry profiling of neoplastic plasma cells in multiple myeloma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The Number of Signaling Pathways Altered by Driver Mutations in Chronic Lymphocytic Leukemia Impacts Disease Outcome

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Machine learning can identify newly diagnosed patients with CLL at high risk of infection

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Distinct immune composition in lymph node and peripheral blood of CLL patients is reshaped during venetoclax treatment

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Ibrutinib and Venetoclax for First-Line Treatment of CLL

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Blodsygdomme

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiFormidling

  • Andy C Rawstron
  • Karl-Anton Kreuzer
  • Asha Soosapilla
  • Martin Spacek
  • Olga Stehlikova
  • Peter Gambell
  • Neil McIver-Brown
  • Neus Villamor
  • Katherina Psarra
  • Maria Arroz
  • Raffaella Milani
  • Javier de la Serna
  • M Teresa Cedena
  • Ozren Jaksic
  • Josep Nomdedeu
  • Carol Moreno
  • Gian Matteo Rigolin
  • Antonio Cuneo
  • Preben Johansen
  • Hans E Johnsen
  • Richard Rosenquist
  • Carsten Utoft Niemann
  • Wolfgang Kern
  • David Westerman
  • Marek Trneny
  • Stephen Mulligan
  • Michael Doubek
  • Sarka Pospisilova
  • Peter Hillmen
  • David Oscier
  • Michael Hallek
  • Paolo Ghia
  • Emili Montserrat
Vis graf over relationer

The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as "required" or "recommended" for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate "required" markers using normal peripheral blood was developed. Responses were received from 150 participants with a diagnostic workload >20 CLL cases per week in 23/150 (15%), 5-20 in 82/150 (55%), and <5 cases per week in 45/150 (30%). The consensus for "required" diagnostic markers included: CD19, CD5, CD20, CD23, Kappa, and Lambda. "Recommended" markers potentially useful for differential diagnosis were: CD43, CD79b, CD81, CD200, CD10, and ROR1. Reproducible criteria for component reagents were assessed retrospectively in 14,643 cases from 13 different centers and showed >97% concordance with current approaches. A pilot study to validate staining quality was completed in 11 centers. Markers considered as "required" for the diagnosis of CLL by the participants in this study (CD19, CD5, CD20, CD23, Kappa, and Lambda) are consistent with current diagnostic criteria and practice. Importantly, a reproducible approach to validate and apply these markers in individual laboratories has been identified. Finally, a consensus "recommended" panel of markers to refine diagnosis in borderline cases (CD43, CD79b, CD81, CD200, CD10, and ROR1) has been defined and will be prospectively evaluated. © 2017 International Clinical Cytometry Society.

OriginalsprogEngelsk
TidsskriftCytometry Part B - Clinical Cytometry
Vol/bind94
Udgave nummer1
Sider (fra-til)121-128
Antal sider8
ISSN1552-4949
DOI
StatusUdgivet - jan. 2018

ID: 52703259