Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Reproducibility of histologic prognostic parameters for mantle cell lymphoma: cytology, Ki67, p53 and SOX11

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Methodological approach to Microscopic Colitis diagnosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Lymphomas of the head and neck region: an update

    Publikation: Bidrag til tidsskriftReviewpeer review

  3. Prognostic significance of 1p36 locus deletion in adenoid cystic carcinoma of the salivary glands

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. The impact of early and successful orchidopexy on hormonal follow-up for 208 boys with bilateral non-syndromic cryptorchidism

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Fertility Potential is Impaired in Boys with Bilateral Ascending Testes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. During infancy low levels of follicle-stimulating hormone may result in high rate of germ cell apoptosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Clinical impact of clonal hematopoiesis in patients with lymphoma undergoing ASCT: a national population-based cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Giorgio A Croci
  • Eva Hoster
  • Sílvia Beà
  • Guillem Clot
  • Anna Enjuanes
  • David W Scott
  • José Cabeçadas
  • Luis Veloza
  • Elias Campo
  • Erik Clasen-Linde
  • Rashmi S Goswami
  • Lars Helgeland
  • Stefano Pileri
  • Grzegorz Rymkiewicz
  • Sarah Reinke
  • Martin Dreyling
  • Wolfram Klapper
Vis graf over relationer

Mantle cell lymphoma (MCL) shows a clinical aggressiveness that varies from patient to patient. Despite major advances in outcomes with current immunochemotherapy, the future development of therapies requires risk stratification to tailor therapy intensity. Within the group of reference pathologists for the ongoing trials of the European MCL Network, we performed a round robin test on a tissue microarray to evaluate the reproducibility in assessing the biomarkers of outcome in MCL. Cytological subtype, Ki67-index and expression of p53 and SOX11 were evaluated on 20 diagnostic tumour samples by eight participating labs independently. We demonstrate that the assessment of the proliferation index by counting the Ki67 positive cells as well as assessment of SOX11 and p53 expression status is reproducible between labs. For the most established prognostic biomarker, Ki67, the intra-class correlation coefficient was very good when assessed as a continuous parameter (0.87). The agreement was lower when the values were analysed in a dichotomized way applying the commonly used cutoff of 30% (kappa = 0.65, complete concordance of all labs in 13/20 (65%)). Cases with discrepant results between labs in the dichotomized analysis showed mean values close to the cutoff of 30%. Centralised scoring and digital image analysis revealed results in line with the scores from individual labs. All cases in our cohort were additionally assessed for gene expression signatures and of TP53 gene alterations. Given the good reproducibility when guidelines of assessment are applied, the biomarker studied in this inter-laboratory test presents potential candidates to be enhanced for risk-stratification in the future clinical trials.

OriginalsprogEngelsk
TidsskriftVirchows Archiv : an international journal of pathology
Vol/bind477
Udgave nummer2
Sider (fra-til)259-267
Antal sider9
ISSN0945-6317
DOI
StatusUdgivet - aug. 2020

ID: 61628686